下丘脑神经内分泌基因表达：原位杂交组织化学研究
Neuroendocrine gene expression in the hypothalamus: in situ hybridization histochemical studies.
作者信息
Young W S, Zoeller R T
机构信息
Laboratory of Cell Biology, National Institute of Mental Health, Bethesda, Maryland 20892.
出版信息
Cell Mol Neurobiol. 1987 Dec;7(4):353-66. doi: 10.1007/BF00733788.
Abstract
- We have reviewed recent studies in which in situ hybridization histochemistry (ISHH) was used to investigate the regulation of expression of neurohypophysial peptides and hypothalamic releasing hormones. 2. ISHH is a technique in which the presence and quantity of a specific mRNA can be determined in tissue sections with a high degree of resolution and sensitivity. 3. ISHH has been used to measure changes in cellular levels of mRNAs encoding vasopressin, oxytocin, corticotropin-releasing factor, gonadotropin-releasing hormone, thyrotropin-releasing hormone and somatostatin in response to various physiological challenges. 4. A theme emerging from these studies is that changes in levels of mRNA encoding neuroendocrine peptides reflect changes in biosynthesis and secretion.
摘要
- 我们回顾了最近的一些研究，这些研究运用原位杂交组织化学（ISHH）来研究神经垂体肽和下丘脑释放激素表达的调控。2. ISHH是一种能够在组织切片中以高分辨率和高灵敏度测定特定mRNA的存在及数量的技术。3. ISHH已被用于测量编码抗利尿激素、催产素、促肾上腺皮质激素释放因子、促性腺激素释放激素、促甲状腺激素释放激素和生长抑素的mRNA细胞水平在各种生理刺激下的变化。4. 这些研究中出现的一个共同主题是，编码神经内分泌肽的mRNA水平的变化反映了生物合成和分泌的变化。
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