Ashtarinezhad Azadeh, Panahyab Ataollah, Mohamadzadehasl Baharak, Shirazi Farshad H
Department of Occupational Health, faculty of Public Health, Iran University of Medical Sciences, Tehran, Iran.
Young Researchers and Elite Club, Central Tehran Branch, Islamic Azad University,Tehran, Iran.
Iran J Pharm Res. 2017 Spring;16(2):677-684.
Azole agents especially Miconazole are widely used even during pregnancy as antifungal agents. The aim of this study was to assess the usefulness of FTIR Micro-Spectroscopy for discriminating of Miconazole treated liver tissue from control liver tissue. The mice were injected with Miconazole (60 mg/Kg) on gestation day 9 and they were dissected on pregnancy day 15. The fetus fixed, dehydrated, and embedded in paraffin. Sections of liver (10 μM) were prepared from control and treated fetus groups by Microtome and deparaffinized with xylene. The spectra were collected using FTIR-MSP in the region of 4000-400 cm. All spectra were normalized to amide II band (1454 cm) after baseline correction of entire spectrum. The results were shown by 2nd derivatization of spectra and also subtracting from control spectra. Miconazole induces some minor changes in the mouse fetus liver at cellular levels when mother is exposed. The most important calculated alterations are in the production of fetus liver proteins. α helical and β sheet structures have shown significant variations, indicating protein alterations configurationally.
唑类药物尤其是咪康唑，即使在孕期也被广泛用作抗真菌剂。本研究的目的是评估傅里叶变换红外显微光谱法在区分咪康唑处理的肝脏组织和对照肝脏组织方面的效用。在妊娠第9天给小鼠注射咪康唑（60毫克/千克），并在妊娠第15天进行解剖。将胎儿固定、脱水并包埋在石蜡中。用切片机从对照和处理过的胎儿组制备肝脏切片（10微米），并用二甲苯脱蜡。使用傅里叶变换红外显微光谱仪在4000 - 400厘米区域收集光谱。在对整个光谱进行基线校正后，将所有光谱归一化为酰胺II带（1454厘米）。结果通过光谱的二阶导数以及从对照光谱中减去来显示。当母体接触咪康唑时，它会在细胞水平上对小鼠胎儿肝脏产生一些微小变化。最重要的计算变化在于胎儿肝脏蛋白质的产生。α螺旋和β折叠结构显示出显著变化，表明蛋白质在构型上发生了改变。
