微波加速固定的神经免疫电子显微镜检查
Neuroimmunological electron microscopy with microwave-accelerated fixation.
作者信息
Armati P J, Pollard J D, Van Reyk D, Van der Lubbe L
机构信息
School of Biological Sciences, University of Sydney, NSW, Australia.
出版信息
J Immunol Methods. 1988 Jun 13;110(2):267-9. doi: 10.1016/0022-1759(88)90113-5.
Immunoelectron microscopy is an important tool used to determine the precise location of immune complexes. Standard concentrations of glutaraldehyde destroy these complexes. This paper describes a method in which the period of glutaraldehyde fixation is shortened by concomitant microwave treatment. Using 1.25% glutaraldehyde and microwave fixation ideal preservation and demonstration of MHC class I antigen on Schwann cells was obtained by the peroxidase method.
免疫电子显微镜是用于确定免疫复合物精确位置的重要工具。标准浓度的戊二醛会破坏这些复合物。本文描述了一种通过同时进行微波处理来缩短戊二醛固定时间的方法。使用1.25%的戊二醛并结合微波固定，通过过氧化物酶法在雪旺细胞上获得了MHC I类抗原的理想保存和显示效果。
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