Zhao Nannan, Xu Yanshan, Wang Kuang, Zheng Suiping
Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology , Guangzhou 510006, P. R. China.
Guangdong Research Center of Industrial Enzyme and Green Manufacturing Technology, School of Biology and Biological Engineering, South China University of Technology , Guangzhou, 510006, P. R. China.
J Agric Food Chem. 2017 Nov 1;65(43):9468-9474. doi: 10.1021/acs.jafc.7b04140. Epub 2017 Oct 23.
We explored the ability of an Aspergillus niger α-glucosidase displayed on P. pastoris to act as a whole-cell biocatalyst (Pp-ANGL-GCW61) system to synthesize isomalto-oligosaccharides (IMOs). IMOs are a mixture that includes isomaltose (IG), panose (P), and isomaltotriose (IG). In this study, the IMOs were synthesized by a hydrolysis-transglycosylation reaction in an aqueous system of maltose. In a 2 mL reaction system, the IMOs were synthesized with a conversion rate of approximately 49% in 2 h when 30% maltose was utilized under optimal conditions by Pp-ANGL-GCW61. Additionally, the 0.5-L reaction system was conducted in a 2-L stirred reactor with a conversion rate of approximately 44% in 2 h. Moreover, the conversion rate was relatively stable after the whole-cell catalyst was reused three times. In conclusion, Pp-ANGL-GCW61 has a high reaction efficiency and operational stability, which makes it a powerful biocatalyst available for industrial scale synthesis.
我们探究了展示在巴斯德毕赤酵母上的黑曲霉α-葡萄糖苷酶作为全细胞生物催化剂（Pp-ANGL-GCW61）系统合成异麦芽寡糖（IMOs）的能力。IMOs是一种包含异麦芽糖（IG）、潘糖（P）和异麦芽三糖（IG）的混合物。在本研究中，IMOs是通过在麦芽糖水体系中的水解转糖基化反应合成的。在2 mL反应体系中，在最佳条件下，当使用30%的麦芽糖时，Pp-ANGL-GCW61在2 h内合成IMOs的转化率约为49%。此外，0.5 L反应体系在2 L搅拌反应器中进行，2 h内转化率约为44%。而且，全细胞催化剂重复使用三次后转化率相对稳定。总之，Pp-ANGL-GCW61具有较高的反应效率和操作稳定性，这使其成为可用于工业规模合成的强大生物催化剂。
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