Department of Microbiology, Government College University, Faisalabad, Pakistan.
College of Allied Health Professionals, Directorate of Medical Sciences, Government College University, Faisalabad, Pakistan.
Future Microbiol. 2017 Nov;12:1261-1269. doi: 10.2217/fmb-2017-0080. Epub 2017 Oct 5.
The ISAba1 insertion sequence supports the overexpression of various oxacillinases genes by providing promoter and therefore, confers carbapenem resistance. The study aimed to determine the clonal diversity, genetic attributes of carbapenem resistance and the role of ISAba1 in the expression of oxacillinases genes among Acinetobacter baumannii clinical isolates.
MATERIALS & METHODS: PCR was performed to amplify various antimicrobial resistance determinants and ISAba1 insertion elements in 137 A. baumannii clinical isolates.
The bla and bla -like genes and ISAba1 upstream to these genes were found in almost all multidrug-resistant isolates belonging to multiple clones.
This is the first comprehensive study from Pakistan that highlighted the emergence of ISAba1 harboring carbapenems resistant A. baumannii stains in tertiary care hospitals.
ISAba1 插入序列通过提供启动子来支持各种耐苯唑西林酶基因的过表达，因此赋予碳青霉烯类耐药性。本研究旨在确定鲍曼不动杆菌临床分离株中碳青霉烯类耐药的克隆多样性、遗传特征以及 ISAba1 在耐苯唑西林酶基因表达中的作用。
对 137 株鲍曼不动杆菌临床分离株进行 PCR 扩增，以扩增各种抗菌药物耐药决定因素和 ISAba1 插入元件。
几乎所有多药耐药株均携带 bla 和 bla-样基因以及这些基因上游的 ISAba1，这些基因属于多个克隆。
这是巴基斯坦首次全面研究，强调了携带 ISAba1 的碳青霉烯类耐药鲍曼不动杆菌菌株在三级医院中的出现。
