Kim Jin Ju, Choi Young Min, Lee Sung Ki, Yang Kwang Moon, Paik Eun Chan, Jeong Hyeon Jeong, Jun Jong Kwan, Han Ae Ra, Hwang Kyu Ri, Hong Min A
a Department of Obstetrics and Gynecology , Healthcare System Gangnam Center, Seoul National University Hospital , Seoul , Korea.
b The Institute of Reproductive Medicine and Population , Medical Research Centre, Seoul National University College of Medicine , Seoul , Korea.
J Obstet Gynaecol. 2018 Feb;38(2):261-264. doi: 10.1080/01443615.2017.1351932. Epub 2017 Oct 5.
Since the first study was published reporting the candidate association between the prolactin receptor gene intron C/T polymorphism (rs37389) and recurrent miscarriage, no replication study has been performed. In this study, we investigated the role of the prolactin receptor gene C/T polymorphism in 311 Korean women with recurrent pregnancy loss and 314 controls. Genotyping for prolactin receptor gene intron C/T polymorphism was performed using a TaqMan assay. The significance of difference in the genotype distribution was assessed using a chi-square test, and continuous variables were compared using a Student's t-test. The genotype distribution of the prolactin receptor gene C/T polymorphism in the recurrent pregnancy loss group did not differ from that in the control group (CC/CT/TT rates were 49.8%/41.5%/8.7% and 52.5%/37.6%/9.9% for the recurrent pregnancy loss patient and control groups, respectively, p = .587). When the analysis was restricted to patients with three or more consecutive spontaneous miscarriages or patients without prior live birth, there were also no differences in the genotype distribution between these subgroups and controls. In conclusion, the findings of the current study suggest that the prolactin receptor gene intron C/T polymorphism is not a major determinant of the development of recurrent pregnancy loss. Impact statement What is already known: Many studies have investigated whether there is a genetic component for the risk of recurrent pregnancy loss. Recently, one study investigated whether genetic polymorphisms involved in the regulation of the hypothalamic-pituitary-ovarian axis would be associated with recurrent miscarriage. Among 35 polymorphisms in 20 candidate genes, genotype distribution with regard to the prolactin receptor gene intron C/T polymorphism (rs37389) differed between the recurrent miscarriage and the control groups. Since this study reporting the candidate association between the prolactin receptor gene and recurrent miscarriage, no replication study has been performed. What the results of this study add: The genotype distribution of the prolactin receptor gene C/T polymorphism in the recurrent miscarriage group did not differ from that in the control group. What the implications are of these findings: Our study may be useful in that it is the first replication study since the initial report of the association of prolactin receptor gene polymorphism with recurrent miscarriage. Although no association was found, the potential role of prolactin in pregnancy loss needs to be further investigated because prolactin and its receptor have been postulated to play an important role in the maintenance of normal pregnancy.
自从首项研究发表，报道催乳素受体基因内含子C/T多态性（rs37389）与复发性流产之间可能存在关联以来，尚未有重复研究进行。在本研究中，我们调查了催乳素受体基因C/T多态性在311名复发性妊娠丢失的韩国女性及314名对照中的作用。采用TaqMan分析法对催乳素受体基因内含子C/T多态性进行基因分型。使用卡方检验评估基因型分布差异的显著性，连续变量则采用学生t检验进行比较。复发性妊娠丢失组中催乳素受体基因C/T多态性的基因型分布与对照组无差异（复发性妊娠丢失患者组和对照组的CC/CT/TT比率分别为49.8%/41.5%/8.7%和52.5%/37.6%/9.9%，p = 0.587）。当分析仅限于有三次或更多次连续自然流产的患者或既往无活产史的患者时，这些亚组与对照组之间的基因型分布也无差异。总之，本研究结果表明，催乳素受体基因内含子C/T多态性并非复发性妊娠丢失发生的主要决定因素。影响声明已知信息：许多研究调查了复发性妊娠丢失风险是否存在遗传因素。最近，一项研究调查了参与下丘脑 - 垂体 - 卵巢轴调节的基因多态性是否与复发性流产有关。在20个候选基因中的35个多态性中，复发性流产组与对照组在催乳素受体基因内含子C/T多态性（rs37389）的基因型分布上存在差异。自从这项报道催乳素受体基因与复发性流产之间可能存在关联的研究以来，尚未有重复研究进行。本研究结果补充了什么：复发性流产组中催乳素受体基因C/T多态性的基因型分布与对照组无差异。这些发现的意义是什么：我们的研究可能有用，因为它是自首次报道催乳素受体基因多态性与复发性流产的关联以来的第一项重复研究。尽管未发现关联，但由于催乳素及其受体被推测在维持正常妊娠中起重要作用，因此催乳素在妊娠丢失中的潜在作用仍需进一步研究。
