Batool R, Salahuddin H, Mahmood T, Ismail M
Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad- 45320, Pakistan.
Institute of Biomedical and Genetic Engineering, Islamabad, Pakistan.
Cell Mol Biol (Noisy-le-grand). 2017 Sep 30;63(9):109-114. doi: 10.14715/cmb/2017.63.9.19.
High-throughput technologies, such as synthetic biology and genomics have paved new paths for discovery and utility of medicinally beneficial plants. Bioactive molecules isolated from different plants have significantly higher biological activities. The present study was done to analyze antibacterial potential of some medicinal plants against multi drug resistant (MDR) pathogens and anticancer effect against MCF-7 cell line. Methanolic and ethanolic extracts were tested for their antibacterial activity by disc diffusion method against six MDR bacterial strains and for cytotoxicity evaluation by MTT assay. Ethanolic extracts of the three tested plants exhibited growth inhibitory effect against Klebsiella pneumonia, Serratia marcescens and Methicillin-resistant S. aureus. Pseudomonas aeruginosa was more resistant to all extracts as its growth was least inhibited by the extracts of all tested plants. Ethanol extract of Foeniculum vulgare exhibited significant inhibition of cancer cells proliferation. Methanol extract of Justicia adhatoda also showed considerable inhibition of cancer cells. Future studies must converge on detailed investigation of modes of action of extracts of tested plants.
高通量技术，如合成生物学和基因组学，为药用植物的发现和利用开辟了新途径。从不同植物中分离出的生物活性分子具有显著更高的生物活性。本研究旨在分析一些药用植物对多重耐药（MDR）病原体的抗菌潜力以及对MCF-7细胞系的抗癌作用。通过纸片扩散法检测甲醇提取物和乙醇提取物对六种MDR细菌菌株的抗菌活性，并通过MTT法进行细胞毒性评估。三种受试植物的乙醇提取物对肺炎克雷伯菌、粘质沙雷氏菌和耐甲氧西林金黄色葡萄球菌具有生长抑制作用。铜绿假单胞菌对所有提取物的耐药性更强，因为所有受试植物的提取物对其生长的抑制作用最小。茴香的乙醇提取物对癌细胞增殖有显著抑制作用。鸭嘴花的甲醇提取物对癌细胞也有相当程度的抑制作用。未来的研究必须集中于对受试植物提取物作用方式的详细研究。
