Reddy S, Bibby N J, Elliott R B
Department of Paediatrics, University of Auckland School of Medicine, New Zealand.
Q J Exp Physiol. 1988 Mar;73(2):225-32. doi: 10.1113/expphysiol.1988.sp003135.
Using antisera to insulin, glucagon, pancreatic polypeptide (PP) and somatostatin, the localization and cellular distribution of the four hormones were investigated in the sheep fetal pancreas of day 40-45 gestation by immunofluorescence. All four hormones were immunolocalized at this early gestational period. The endocrine cell types had a characteristic distribution and were present in different numbers. Insulin and glucagon immunoreactive cells were seen in larger numbers compared to fetal PP and somatostatin cells and were located either in the developing islets or as single scattered cells in the epithelium of the embryonic ductules. These cells became more confined to the developing islets at later stages of gestation. In the pancreas of day 40-45 fetuses PP cells were less numerous than glucagon and insulin cells while somatostatin cells were seen rarely. However, PP and somatostatin cells became more numerous at later stages of gestation. Our studies demonstrate the presence of insulin, glucagon, PP and somatostatin within distinct cell types in the early sheep fetal pancreas.
利用抗胰岛素、胰高血糖素、胰多肽（PP）和生长抑素的抗血清，通过免疫荧光法研究了妊娠40 - 45天绵羊胎儿胰腺中这四种激素的定位和细胞分布。在这个妊娠早期，所有四种激素都被免疫定位。内分泌细胞类型具有特征性分布且数量不同。与胎儿PP细胞和生长抑素细胞相比，胰岛素和胰高血糖素免疫反应性细胞数量更多，它们位于发育中的胰岛中，或者作为单个散在细胞存在于胚胎小导管的上皮中。在妊娠后期，这些细胞更多地局限于发育中的胰岛。在妊娠40 - 45天胎儿的胰腺中，PP细胞比胰高血糖素和胰岛素细胞数量少，而生长抑素细胞很少见。然而，PP细胞和生长抑素细胞在妊娠后期数量增多。我们的研究表明，在早期绵羊胎儿胰腺的不同细胞类型中存在胰岛素、胰高血糖素、PP和生长抑素。
