School of Environmental Science and Engineering and Institute of Environmental Health and Pollution Control, Guangdong University of Technology, 510006 Guangzhou, PR China.
School of Environmental Science and Engineering and Institute of Environmental Health and Pollution Control, Guangdong University of Technology, 510006 Guangzhou, PR China.
Bioresour Technol. 2018 Jan;247:591-598. doi: 10.1016/j.biortech.2017.09.147. Epub 2017 Sep 22.
This work aims at establishing a coupling anaerobic-aerobic biofilm within a single bioreactor and revealing its microbial community and succession. By using a semi-suspended bio-carrier fabricated with 3D printing technique, an obvious DO gradient was gradually created within the biofilm, which demonstrated that a coupling anaerobic-aerobic biofilm was successfully established on the surface of bio-carriers. The results of metagenomic analysis revealed that the microbial community on the bio-carriers experienced a continuous succession in its structure and dominant species along with the operational time. The formed coupling biofilm created suitable micro multi-habitats for the co-existence of these microorganisms, including nitrifying and denitrifying bacteria, which were beneficial to the removing of organic pollutants and converting nutrients. Along with the succession, the microbial community was gradually dominated by several functional microorganisms. Overall, the results presented an approach to improve the microbial biodiversity by constructing a new structure and floating status of bio-carriers.
本工作旨在建立一种在单个生物反应器内的厌氧-好氧耦合生物膜，并揭示其微生物群落和演替过程。通过使用 3D 打印技术制造的半悬浮生物载体，在生物膜内逐渐形成明显的 DO 梯度，证明在生物载体表面成功建立了厌氧-好氧耦合生物膜。宏基因组分析的结果表明，随着运行时间的延长，生物载体上的微生物群落结构和优势物种不断发生演替。形成的耦合生物膜为这些微生物的共存创造了合适的微多生境，包括硝化和反硝化细菌，这有利于去除有机污染物和转化营养物质。随着演替的进行，微生物群落逐渐被少数几种功能微生物所主导。总的来说，该结果提供了一种通过构建生物载体的新结构和悬浮状态来提高微生物生物多样性的方法。
