Shenzhen Research Institute, School of Environmental Science and Engineering, Shandong University, PR China; College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, PR China; School of Environmental Science and Engineering, Shanghai Jiaotong University, PR China.
Shenzhen Research Institute, School of Environmental Science and Engineering, Shandong University, PR China.
Bioresour Technol. 2018 Jan;247:259-266. doi: 10.1016/j.biortech.2017.09.070. Epub 2017 Sep 19.
Membrane bioreactor with non-woven fabric membranes (NWMBR) is developing into a suitable method for anaerobic ammonium oxidation (anammox). As a carrier, non-woven fabric membrane divided total biomass into biofilm and suspended flocs gradually. Total nitrogen removal efficiency was maintained around 82.6% under nitrogen loading rate of 567.4mgN/L/d after 260days operation. Second-order substrate removal and Stover-Kincannon models were successfully used to simulate the nitrogen removal performance in NWMBR. High-throughput sequence was employed to elucidate the underlying microbial community dynamics. Candidatus Brocadia, Kuenenia, Jettenia were detected to affirm the dominant status of anammox microorganisms and 98.2% of anammox microorganisms distributed in biofilm. In addition, abundances of functional genes (hzs, nirK) in biofilm and suspended flocs were assessed by quantitative PCR to further investigate the coexistence of anammox and other microorganisms. Potential nitrogen removal pathways were established according to relevant nitrogen removal performance and microbial community.
采用无纺膜（NWMBR）的膜生物反应器正逐渐发展成为一种适合厌氧氨氧化（Anammox）的方法。作为载体，无纺膜逐渐将总生物质分为生物膜和悬浮絮体。经过 260 天的运行，在氮负荷为 567.4mgN/L/d 的条件下，总氮去除效率保持在 82.6%左右。采用二级底物去除和 Stover-Kincannon 模型成功模拟了 NWMBR 的氮去除性能。高通量测序用于阐明潜在的微生物群落动态。检测到疑似布鲁氏菌、昆尼氏菌和杰滕氏菌，以确认厌氧氨氧化微生物的优势地位，98.2%的厌氧氨氧化微生物分布在生物膜中。此外，通过定量 PCR 评估生物膜和悬浮絮体中功能基因（hzs、nirK）的丰度，进一步研究厌氧氨氧化菌与其他微生物的共存情况。根据相关氮去除性能和微生物群落，建立了潜在的氮去除途径。
