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一种简单的方法,可对果蝇中复杂染色体结构的大规模核型分析。

An easy route to the massive karyotyping of complex chromosomal arrangements in Drosophila.

机构信息

Departament de Genètica, Microbiologia i Estadística, Facultat de Biologia and Institut de Recerca de la Biodiversitat (IRBio), Universitat de Barcelona, Diagonal 643, 08028, Barcelona, Spain.

出版信息

Sci Rep. 2017 Oct 5;7(1):12717. doi: 10.1038/s41598-017-13043-6.

DOI:10.1038/s41598-017-13043-6
PMID:28983092
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5629216/
Abstract

Inversion polymorphism is widespread in the Drosophila genus as well as in other dipteran genera. The presence of polytene chromosomes in some insect organs and, thus, the possibility to observe the different arrangements generated by inversions through a microscope enhanced the cytological study of this structural polymorphism. In several Drosophila species, these studies provided evidence for the adaptive character of this polymorphism, which together with the standing interest to uncover targets of natural selection has led to a renewed interest for inversion polymorphism. Our recent molecular characterization of the breakpoint regions of five inversions of the E chromosome of D. subobscura has allowed us to design a PCR-based strategy to molecularly identify the different chromosomal arrangements and, most importantly, to determine the E chromosome karyotype of medium- and large-sized samples from natural populations. Individuals of a test sample that were both cytologically and molecularly karyotyped were used to establish the strategy that was subsequently applied to karyotype a larger sample. Our strategy has proved to be robust and time efficient, and it lays therefore the groundwork for future studies of the E chromosome structural polymorphism through space and time, and of its putative contribution to adaptation.

摘要

倒位多态性在果蝇属以及其他双翅目属中广泛存在。一些昆虫器官中存在多线染色体,因此可以通过显微镜观察倒位产生的不同排列,这增强了对这种结构多态性的细胞学研究。在一些果蝇物种中,这些研究为这种多态性的适应性特征提供了证据,这与揭示自然选择目标的持续兴趣一起,导致了对倒位多态性的重新关注。我们最近对 D. subobscura 的 E 染色体的五个倒位的断点区域进行了分子特征分析,这使我们能够设计一种基于 PCR 的策略,对不同的染色体排列进行分子鉴定,最重要的是,确定来自自然种群的中大型样本的 E 染色体核型。对同时进行细胞学和分子核型分析的测试样本个体进行了使用,以建立随后应用于更大样本的核型分析的策略。我们的策略被证明是稳健和高效的,因此为未来通过空间和时间研究 E 染色体结构多态性及其对适应性的可能贡献奠定了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa4a/5629216/ce7c1eadf31f/41598_2017_13043_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa4a/5629216/0f3883bf2319/41598_2017_13043_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa4a/5629216/d9aabf85e5a7/41598_2017_13043_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa4a/5629216/ce7c1eadf31f/41598_2017_13043_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa4a/5629216/0f3883bf2319/41598_2017_13043_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa4a/5629216/d9aabf85e5a7/41598_2017_13043_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa4a/5629216/ce7c1eadf31f/41598_2017_13043_Fig3_HTML.jpg

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引用本文的文献

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本文引用的文献

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