Wang Dandan, Huang Zhi, He Hong, Wei Cong
Key Laboratory of Plant Protection Resources and Pest Management, Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.
College of Forestry, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.
Arch Microbiol. 2018 Mar;200(2):227-235. doi: 10.1007/s00203-017-1432-8. Epub 2017 Oct 5.
Plant sap-feeding insects of Hemiptera often form intimate symbioses with microbes to obtain nutrients. The cicada Subpsaltria yangi is the only species of the subfamily Tettigadinae known from China. Using high-throughput sequencing combined with fluorescence in situ hybridization analysis, we characterize the bacterial composition of the bacteriomes, testes, ovaries and eggs of two representative populations of this species which occur in different habitats and feed on different plant hosts. In both populations, the bacterial community diversity in the testes was significantly higher than that in other tissues. The obligate endosymbiont Candidatus Sulcia muelleri was observed in all samples and was dominant in the bacteriomes, ovaries and eggs. The usual co-resident endosymbiont Candidatus Hodgkinia cicadicola found in some other cicadas was not detected. Instead, a novel Rhizobiales bacterium which shows a ~ 81% 16S rDNA similarity to Ca. Hodgkinia cicadicola was detected. Given that the genome of Ca. Hodgkinia cicadicola exhibits rapid evolution, it is possible that this novel Rhizobiales bacterium is a related endosymbiont with beneficial trophic functions similar to that of Ca. Hodgkinia cicadicola hosted by several certain other cicadas. The presence of the novel Rhizobiales species in other cicadas and its involvement with the adaptive evolution of related cicada hosts require further investigation. Discrepancy of bacterial communities associated with testes between the two populations may be closely related to the geographic isolation and divergence of habitats and host plants. Our results are informative for further studies of evolutionary divergence of related endosymbionts hosted in cicadas.
半翅目吸食植物汁液的昆虫通常与微生物形成紧密的共生关系以获取营养。杨氏副喙蝉是中国已知的喙蝉亚科唯一物种。我们采用高通量测序结合荧光原位杂交分析，对该物种两个分布于不同栖息地、取食不同植物寄主的代表性种群的菌胞、睾丸、卵巢和卵的细菌组成进行了表征。在两个种群中，睾丸中的细菌群落多样性均显著高于其他组织。在所有样本中均观察到专性内共生菌穆氏念珠杆菌，且在菌胞、卵巢和卵中占主导地位。在其他一些蝉中常见的共居内共生菌霍氏卡迪基尼亚菌未被检测到。相反，检测到一种新的根瘤菌目细菌，其16S rDNA与霍氏卡迪基尼亚菌的相似度约为81%。鉴于霍氏卡迪基尼亚菌的基因组呈现快速进化，这种新的根瘤菌目细菌有可能是一种相关内共生菌，具有与其他几种蝉所携带的霍氏卡迪基尼亚菌类似的有益营养功能。该新根瘤菌目物种在其他蝉中的存在及其与相关蝉寄主适应性进化的关系有待进一步研究。两个种群中与睾丸相关的细菌群落差异可能与地理隔离以及栖息地和寄主植物的分化密切相关。我们的研究结果为进一步研究蝉所携带的相关内共生菌的进化分歧提供了信息。
