Zhou Xing-Kui, Li Qing-Qing, Mo Ming-He, Zhang Yong-Guang, Dong Li-Min, Xiao Min, Li Wen-Jun, Duan Yan-Qing
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming 650091, PR China.
Life Science College, Southwest Forestry University, Kunming 650224, PR China.
Int J Syst Evol Microbiol. 2017 Nov;67(11):4808-4813. doi: 10.1099/ijsem.0.002381. Epub 2017 Oct 6.
A Gram-staining-negative, non-motile and rod-shaped bacterium, designated strain h337, was isolated from an arable soil sample of a tobacco field in Kunming, south-west China. The cells showed oxidase-positive and catalase-positive reactions. Growth was observed at 10-35 °C, at pH 6.0-9.0 and in the presence of up to 3 % (w/v) NaCl, with optimal growth at 30 °C, pH 7.0 and with 1-2 % (w/v) NaCl. The predominant isoprenoid quinone was MK-7. The major fatty acids were identified as iso-C15 : 0, iso-C17 : 0 3-OH, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B). The cellular polar lipids contained phosphatidylethanolamine, sphingophospholipid, four unidentified phospholipids, five unidentified lipids and three unidentified aminophospholipids. The genomic DNA G+C content was 41.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain h337 should be assigned to the genus Sphingobacterium. 16S rRNA gene sequence similarity analysis showed that strain h337 was most closely related to 'Sphingobacteriumyamdrokense' 3-0-1 (98.8 %) and Sphingobacteriumyanglingense CCNWSP36-1 (98.5 %) and shared less than 97 % similarity with other species of the genus Sphingobacterium. DNA-DNA hybridization data indicated that the isolate represented a novel genomic species belonging to the genus Sphingobacterium. The characteristics determined in this polyphasic taxonomic study indicated that strain h337 represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium tabacisoli sp. nov. (type strain h337=KCTC 52298=CCTCC AB 2017155) is proposed.
从中国西南部昆明市一块烟草田的耕地土壤样本中分离出一株革兰氏染色阴性、无运动性的杆状细菌，命名为菌株h337。该菌细胞氧化酶反应呈阳性，过氧化氢酶反应也呈阳性。在10-35 °C、pH 6.0-9.0以及存在高达3%（w/v）NaCl的条件下可观察到其生长，最适生长温度为30 °C，pH 7.0，NaCl浓度为1-2%（w/v）。主要的类异戊二烯醌为MK-7。主要脂肪酸鉴定为异-C15 : 0、异-C17 : 0 3-OH、特征性总和3（C16 : 1ω7c和/或C16 : 1ω6c）以及特征性总和4（异-C17 : 1 I和/或anteiso-C17 : 1 B）。细胞极性脂质包含磷脂酰乙醇胺、鞘磷脂、四种未鉴定的磷脂、五种未鉴定的脂质以及三种未鉴定的氨基磷脂。基因组DNA G+C含量为41.5 mol%。基于16S rRNA基因序列的系统发育分析表明，菌株h337应归属于鞘氨醇杆菌属。16S rRNA基因序列相似性分析表明，菌株h337与“亚东鞘氨醇杆菌”3-0-1（98.8%）和杨凌鞘氨醇杆菌CCNWSP36-1（98.5%）关系最为密切，与鞘氨醇杆菌属的其他物种相似度低于97%。DNA-DNA杂交数据表明，该分离株代表鞘氨醇杆菌属的一个新基因组种。在这项多相分类研究中确定的特征表明，菌株h337代表鞘氨醇杆菌属的一个新物种，为此提出名为烟草鞘氨醇杆菌新种（模式菌株h337=KCTC 52298=CCTCC AB 2017155）。
