Fu Yu-Song, Hussain Firasat, Habib Neeli, Khan Inam Ullah, Chu Xiao, Duan Yan-Qing, Zhi Xiao-Yang, Chen Xing, Li Wen-Jun
Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming, 650091, PR China.
China Tobacco Yunnan Industrial Co. Ltd, Kunming, 650231, PR China.
Int J Syst Evol Microbiol. 2017 Jul;67(7):2284-2288. doi: 10.1099/ijsem.0.001946. Epub 2017 Jul 12.
A Gram-stain-negative, strictly aerobic, rod-shaped, non-motile, non-spore-forming bacterial strain, designated YIM X0211T, was isolated from a soil sample of Shiling County, Yunnan Province, south-west China. The new isolate was characterized taxonomically by using a polyphasic approach. The strain grew optimally at 30 °C, at pH 7.0 and with 0-3 % (w/v) NaCl. It was positive for catalase and oxidase but negative for H2S production. Comparative 16S rRNA gene sequence analysis showed that strain YIM X0211T fell within the cluster comprising Sphingobacterium species and clustered with Sphingobacterium mizutaii DSM 11724T (97.93 % similarity). The G+C content of the genomic DNA was 41.2 mol%. The predominant respiratory quinone was menaquinone MK-7. The major fatty acids were iso-C15 : 0 2-OH, iso-C17 : 0 3-OH and summed feature 3 (C16 : 1ω7c/C16 : 1ω6c). The polar lipids consisted of phosphatidylethanolamine, sphingolipid, and several unknown phospholipids or lipids. The DNA-DNA hybridization value between strain YIM X0211T and S. mizutaii DSM 11724T was 42.3±0.4 %, which is below the 70 % limit for species delineation. These chemotaxonomic data supported the affiliation of strain YIM X0211T to the genus Sphingobacterium. Based on the recorded phenotypic and genotypic characteristics, it is determined that the isolate represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium soli sp. nov. is proposed. The type strain is YIM X0211T (=KCTC 42696T=CGMCC 1.15966T).
从中国西南部云南省石林县的一份土壤样品中分离出一株革兰氏染色阴性、严格需氧、杆状、无运动性、不产芽孢的细菌菌株，命名为YIM X0211T。采用多相分类方法对该新分离菌株进行了分类学特征分析。该菌株在30 °C、pH 7.0和0-3 %（w/v）NaCl条件下生长最佳。过氧化氢酶和氧化酶检测呈阳性，但硫化氢产生检测呈阴性。比较16S rRNA基因序列分析表明，菌株YIM X0211T属于鞘氨醇杆菌属菌群，与水田鞘氨醇杆菌DSM 11724T聚类（相似性为97.93 %）。基因组DNA的G+C含量为41.2 mol%。主要呼吸醌为甲基萘醌MK-7。主要脂肪酸为异-C15 : 0 2-OH、异-C17 : 0 3-OH和特征性总和3（C16 : 1ω7c/C16 : 1ω6c）。极性脂质由磷脂酰乙醇胺、鞘脂以及几种未知的磷脂或脂质组成。菌株YIM X0211T与水田鞘氨醇杆菌DSM 11724T之间的DNA-DNA杂交值为42.3±0.4 %，低于物种界定的70 %界限。这些化学分类学数据支持菌株YIM X0211T属于鞘氨醇杆菌属。基于所记录的表型和基因型特征，确定该分离株代表鞘氨醇杆菌属的一个新物种，为此提出新名称土壤鞘氨醇杆菌（Sphingobacterium soli sp. nov.）。模式菌株为YIM X0211T（=KCTC 42696T=CGMCC 1.15966T）。
