移植到卵泡期和黄体期牛卵泡中的牛卵母细胞的成熟。
Maturation of bovine oocytes transplanted into bovine follicles of follicular and luteal phase.
作者信息
Gerritse R, Kruip T A, van Beneden T H, te Velde E R
机构信息
Academic Hospital, Department of Obstetrics and Gynaecology A.L., University of Utrecht, The Netherlands.
出版信息
Int J Fertil. 1988 Mar-Apr;33(2):134-8.
Forty-one bovine host follicles with guest oocytes were studied. Fifty of these host follicles from the follicular phase and 26 follicles from the luteal phase of the cycle were cultured in a continuous flux system. No hormones were added to the follicular fluid or the culture medium. After culturing there was no close contact between the oocyte complex and the follicular wall. Of the oocytes cultured in the follicular-phase follicles, 27.2% remained in the germinal vesicle stage and 7.1% reached MII, whereas 9.9% of the oocytes cultured in the luteal-phase follicles remained in germinal vesicle phase and 29.5% reached MII. Of the oocytes cultured in the luteal-phase host follicles, 15.2% were fertilized in vitro; none of the oocytes cultured in follicular-phase follicles were fertilized.
对41个含有寄生卵母细胞的牛宿主卵泡进行了研究。从卵泡期选取50个这些宿主卵泡，从周期的黄体期选取26个卵泡，在连续通量系统中进行培养。卵泡液或培养基中未添加任何激素。培养后，卵母细胞复合体与卵泡壁之间没有紧密接触。在卵泡期卵泡中培养的卵母细胞，27.2% 停留在生发泡期，7.1% 达到减数第二次分裂中期；而在黄体期卵泡中培养的卵母细胞，9.9% 停留在生发泡期，29.5% 达到减数第二次分裂中期。在黄体期宿主卵泡中培养的卵母细胞，15.2% 在体外受精；在卵泡期卵泡中培养的卵母细胞均未受精。
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