Lysobacter olei sp. nov., isolated from oil-contaminated soil.

Strain D-14, a brown-coloured, Gram-stain-negative, non-motile and rod-shaped bacterium, was isolated from oil-contaminated soil. It was able to grow at 20-40 °C, at pH 6.0-10.0 and at 0-1 % (w/v) NaCl concentration. Based on the 16S rRNA gene sequence analysis, strain D-14 belonged to the genus Lysobacter and was closely related to Lysobacter caeni BUT-8 (99.0 % sequence similarity), Lysobacter ruishenii CTN-1 (98.5 %), Lysobacter daejeonensis GH1-9 (98.2 %) and Lysobacter panacisoli CJ29 (97.2 %). The only respiratory quinone was ubiquinone-8. The polar lipid profile revealed the presence of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidyl-N-methyl-ethanolamine. The predominant fatty acids of strain D-14 were iso-C15 : 0, iso-C16 : 0, summed feature 9 (iso-C17 : 1 ω9c and/or C16 : 0 10-methyl), summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c), iso-C14 : 0, C11 : 0iso 3-OH, C15 : 1iso F and C16 : 0. The genomic DNA G+C content of this novel strain was 68.7 mol%. The DNA-DNA relatedness values between strain D-14 and L. caeni BUT-8, L. ruishenii CTN-1, L. daejeonensis GH1-9 and L. panacisoli CJ29 were 56.0, 46.3, 48.7 and 41.7 %, respectively, which fall below the threshold value of 70 % for the strain to be considered as novel. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours. Thus, strain D-14 represents a novel species of the genus Lysobacter, for which the name Lysobacterolei sp. nov. is proposed. The type strain is D-14 (=KEMB 9005-572=KACC 19173=JCM 31917).