Chaudhary Dhiraj Kumar, Lee Sang Don, Kim Jaisoo
Department of Life Science, College of Natural Sciences and Engineering, Kyonggi University, Suwon, Gyeonggi-Do 16227, Republic of Korea.
Department of Environmental Science and Engineering, College of Engineering, Ewha Womans University, Seoul 03760, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Nov;67(11):4660-4666. doi: 10.1099/ijsem.0.002348. Epub 2017 Oct 6.
Strain D-14, a brown-coloured, Gram-stain-negative, non-motile and rod-shaped bacterium, was isolated from oil-contaminated soil. It was able to grow at 20-40 °C, at pH 6.0-10.0 and at 0-1 % (w/v) NaCl concentration. Based on the 16S rRNA gene sequence analysis, strain D-14 belonged to the genus Lysobacter and was closely related to Lysobacter caeni BUT-8 (99.0 % sequence similarity), Lysobacter ruishenii CTN-1 (98.5 %), Lysobacter daejeonensis GH1-9 (98.2 %) and Lysobacter panacisoli CJ29 (97.2 %). The only respiratory quinone was ubiquinone-8. The polar lipid profile revealed the presence of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidyl-N-methyl-ethanolamine. The predominant fatty acids of strain D-14 were iso-C15 : 0, iso-C16 : 0, summed feature 9 (iso-C17 : 1 ω9c and/or C16 : 0 10-methyl), summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c), iso-C14 : 0, C11 : 0iso 3-OH, C15 : 1iso F and C16 : 0. The genomic DNA G+C content of this novel strain was 68.7 mol%. The DNA-DNA relatedness values between strain D-14 and L. caeni BUT-8, L. ruishenii CTN-1, L. daejeonensis GH1-9 and L. panacisoli CJ29 were 56.0, 46.3, 48.7 and 41.7 %, respectively, which fall below the threshold value of 70 % for the strain to be considered as novel. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours. Thus, strain D-14 represents a novel species of the genus Lysobacter, for which the name Lysobacterolei sp. nov. is proposed. The type strain is D-14 (=KEMB 9005-572=KACC 19173=JCM 31917).
菌株D-14是一种从石油污染土壤中分离出的革兰氏染色阴性、无运动性的棕色杆状细菌。它能够在20-40°C、pH值6.0-10.0以及0-1%（w/v）NaCl浓度下生长。基于16S rRNA基因序列分析，菌株D-14属于溶杆菌属，与嗜碱溶杆菌BUT-8（序列相似性99.0%）、鲁氏溶杆菌CTN-1（98.5%）、大田溶杆菌GH1-9（98.2%）和人参根际溶杆菌CJ29（97.2%）密切相关。唯一的呼吸醌是泛醌-8。极性脂质谱显示存在磷脂酰乙醇胺、磷脂酰甘油、二磷脂酰甘油和磷脂酰-N-甲基乙醇胺。菌株D-14的主要脂肪酸为异C15:0、异C16:0、总和特征9（异C17:1 ω9c和/或C16:0 10-甲基）、总和特征3（C16:1 ω7c和/或C16:1 ω6c）、异C14:0、C11:0iso 3-OH、C15:1iso F和C16:0。该新菌株的基因组DNA G+C含量为68.7 mol%。菌株D-14与嗜碱溶杆菌BUT-8、鲁氏溶杆菌CTN-1、大田溶杆菌GH1-9和人参根际溶杆菌CJ29之间的DNA-DNA相关性值分别为56.0%、46.3%、48.7%和41.7%，均低于将该菌株视为新物种所需的70%阈值。形态学、生理学、化学分类学和系统发育分析清楚地将该菌株与其最密切的系统发育邻居区分开来。因此，菌株D-14代表溶杆菌属的一个新物种，为此提出名称溶油溶杆菌（Lysobacterolei sp. nov.）。模式菌株为D-14（=KEMB 9005-572=KACC 19173=JCM 31917）。
