Ye Xiao-Mei, Chu Cui-Wei, Shi Chao, Zhu Jian-Chun, He Qin, He Jian
The Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, PR China.
Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
Int J Syst Evol Microbiol. 2015 Mar;65(Pt 3):845-850. doi: 10.1099/ijs.0.000024. Epub 2014 Dec 15.
Strain BUT-8(T), a Gram-stain-negative, non-motile and rod-shaped aerobic bacterium, was isolated from the activated sludge of a herbicide-manufacturing wastewater treatment facility. Comparative 16S rRNA gene sequence analysis revealed that strain BUT-8(T) clustered with species of the genus Lysobacter and was closely related to Lysobacter ruishenii DSM 22393(T) (98.3 %) and Lysobacter daejeonensis KACC 11406(T) (98.7 %). The DNA G+C content of the genomic DNA was 70.6 mol%. The major respiratory quinone was ubiquinone-8, and the major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an aminolipid. The major cellular fatty acids were iso-C15 : 0, iso-C16 : 0, iso-C17 : 0, iso-C11 : 0, iso-C11 : 0 3OH and summed feature 9 (comprising iso-C17 : 1ω9c and/or C16 : 010-methyl). The DNA-DNA relatedness between strain BUT-8(T) and its closest phylogenetic neighbours was below 70 %. Phylogenetic, chemotaxonomic and phenotypic results clearly demonstrated that strain BUT-8(T) belongs to the genus Lysobacter and represents a novel species for which the name Lysobacter caeni sp. nov. is proposed. The type strain is BUT-8(T) ( = CCTCC AB 2013087(T) = KACC 17141(T)).
菌株BUT-8(T)是一种革兰氏染色阴性、无运动性的杆状需氧细菌，从一家除草剂制造废水处理设施的活性污泥中分离得到。16S rRNA基因序列比较分析表明，菌株BUT-8(T)与溶杆菌属的物种聚类，与鲁氏溶杆菌DSM 22393(T)（98.3%）和大田溶杆菌KACC 11406(T)（98.7%）密切相关。基因组DNA的G+C含量为70.6 mol%。主要呼吸醌为泛醌-8，主要极性脂为双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油和一种氨基脂。菌株BUT-8(T)与其最接近的系统发育邻居之间的DNA-DNA相关性低于70%。系统发育、化学分类和表型结果清楚地表明，菌株BUT-8(T)属于溶杆菌属，代表一个新物种，为此提出了新名称嗜泥溶杆菌（Lysobacter caeni sp. nov.）。模式菌株为BUT-8(T)（=CCTCC AB 2013087(T)=KACC 17141(T)）。
