Kineococcus terrestris sp. nov. and Kineococcus aureolus sp. nov., isolated from saline sediment.

Two novel actinobacteria, designated YIM 121936 and YIM 121940, were isolated from alkaline sediment in Yuanjiang, China. The cells of the novel strains were Gram-stain-positive, aerobic, motile, non-spore-forming and coccus-shaped. The two strains both contained meso-diaminopimelic acid as the diagnostic diamino acid. The whole-cell sugars were arabinose, galactose, glucose, mannose and ribose. The predominant menaquinone was MK-9(H2). The polar lipid profile of both strains comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, one unknown phosphoglycolipid and five unknown phospholipids. The predominant fatty acids of both strains were anteiso-C15 : 0. The genomic DNA G+C contents of strains YIM 121936 and YIM 121940 were 74.7 and 75.2 %, respectively. Strain YIM 121936 was closely related to Kineococcus aurantiacus IFO 15268 (97.19 %), Kineococcus gypseus YIM 121300 (97.00 %) and Kineococcus mangrovi NBRC 110933 (97.00 %). Strain YIM 121940 was closely related to K. aurantiacus IFO 15268 (97.41 %), Kineococcus endophytica KLBMP 1274 (97.18 %), Kineococcus rhizosphaerae RP-B16 (97.09 %), Kineococcus radiotolerans SRS 30216 (97.09 %), K. gypseus YIM 121300 (97.00 %) and K. mangrovi NBRC 110933 (97.00 %). Strain YIM 121936 shared high 16S rRNA gene sequence similarity (99 %) with YIM 121940. Similarities of two strains with other species of the genus Kineococcus were <97 %. The DNA-DNA hybridization values were below 70 % among all the strains studied. YIM 121936 and YIM 121940 are representatives of two new species in the genus Kineococcus, for which names Kineococcus terreus sp. nov. (type strain YIM 121936=KCTC 39738=DSM 102155) and Kineococcus aureolus sp. nov. (type strain YIM 121940=KCTC 39739=DSM 102158) are proposed, respectively.