Wang Bin, Xu Xiao-Bing, Jin Xin-Xin, Wu Xiao-Wei, Li Min-Li, Guo Mei-Xia, Zhang Xiao-Hua
From the Department of Gastroenterology, Jinling Hospital, Nanjing, China.
Pancreas. 2017 Nov/Dec;46(10):1267-1274. doi: 10.1097/MPA.0000000000000935.
The aims of this study were to determine the effects of ω-3 fatty acids (ω-3FAs) on the Toll-like receptor 4 (TLR4)/nuclear factor κB p56 (NF-κBp56) signaling pathway in the pancreas of rats with severe acute pancreatitis (SAP).
Sixty-four Sprague-Dawley rats were randomly divided into 4 groups: the control, SAP-saline, SAP-soybean oil, and SAP-ω-FA groups. Severe acute pancreatitis was induced by the retrograde infusion of sodium taurocholate into the pancreatic duct. The expression of TLR4 and NF-κBp56 in the pancreas was evaluated by immunohistochemistry and Western blot analysis. The levels of the proinflammatory cytokines interleukin 6 and tumor necrosis factor α in the pancreas were measured by enzyme-linked immunosorbent assays.
Toll-like receptor 4, NF-κBp56, and inflammatory cytokine expression in the pancreas was increased significantly in the SAP group compared with that in the control group (P < 0.05), but was significantly decreased in the ω-3FA group compared with that in the soybean oil group at 24 and 48 hours (P < 0.05).
Our results suggest that during the initial stage of SAP ω-3FAs could efficiently lower the inflammatory response by activating the TLR4/NF-κBp56 signaling pathway.
本研究旨在确定ω-3脂肪酸（ω-3FAs）对重症急性胰腺炎（SAP）大鼠胰腺中Toll样受体4（TLR4）/核因子κB p56（NF-κBp56）信号通路的影响。
将64只Sprague-Dawley大鼠随机分为4组：对照组、SAP-生理盐水组、SAP-大豆油组和SAP-ω-脂肪酸组。通过向胰管逆行注入牛磺胆酸钠诱导重症急性胰腺炎。采用免疫组织化学和蛋白质印迹分析评估胰腺中TLR4和NF-κBp56的表达。采用酶联免疫吸附测定法测量胰腺中促炎细胞因子白细胞介素6和肿瘤坏死因子α的水平。
与对照组相比，SAP组胰腺中Toll样受体4、NF-κBp56和炎性细胞因子的表达显著增加（P<0.05），但在24小时和48小时时，与大豆油组相比，ω-3脂肪酸组显著降低（P<0.05）。
我们的结果表明，在SAP的初始阶段，ω-3FAs可通过激活TLR4/NF-κBp56信号通路有效降低炎症反应。
