Wang Geng Nan, Wu Ning Peng, He Xin, Zhang Hui Cai, Liu Jing, Wang Jian Ping
College of Veterinary Medicine, Agricultural University of Hebei, Baoding, Hebei, 071000, China.
Henan Institute of Veterinary Drug Control, Zhengzhou, Henan, 450002, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Nov 1;1067:18-24. doi: 10.1016/j.jchromb.2017.09.043. Epub 2017 Sep 28.
In this study, a magnetic graphene-based dispersive solid phase extraction method was first developed for extraction of β-agonists in urine. During the experiments, the absorbent amount, sample pH, extraction time, elution solution and elution time were optimized respectively. The optimized extraction method was finished within 10min, and showed high enrichment factors for 9 β-agonists (20-26 folds). Furthermore, this absorbent could be reused for at least 60 times. Then this extraction method was combined with ultra performance liquid chromatography triple quadrupole tandem mass spectrometry to determine the 9 drugs in urine. The limits of detection for the 9 drugs were in a range of 0.015-0.023ngmL, and the recoveries from the standards fortified blank urine were in a range of 60.2%-109.4%. Therefore, this method could be used as a simple, rapid, sensitive and accurate tool to determine trace level of β-agonists in urine.
在本研究中，首次开发了一种基于磁性石墨烯的分散固相萃取方法用于尿液中β-激动剂的萃取。实验过程中，分别对吸附剂用量、样品pH值、萃取时间、洗脱溶液和洗脱时间进行了优化。优化后的萃取方法在10分钟内完成，对9种β-激动剂显示出较高的富集倍数（20 - 26倍）。此外，这种吸附剂可重复使用至少60次。然后将该萃取方法与超高效液相色谱三重四极杆串联质谱联用，用于测定尿液中的9种药物。这9种药物的检测限在0.015 - 0.023 ng/mL范围内，加标空白尿液的回收率在60.2% - 109.4%范围内。因此，该方法可作为一种简单、快速、灵敏且准确的工具来测定尿液中痕量水平的β-激动剂。
