He Xin, Wang Geng Nan, Yang Kun, Liu Hui Zhi, Wu Xia Jun, Wang Jian Ping
College of Veterinary Medicine, Agricultural University of Hebei, Baoding, Hebei 071000 China.
College of Veterinary Medicine, Agricultural University of Hebei, Baoding, Hebei 071000 China.
Food Chem. 2017 Apr 15;221:1226-1231. doi: 10.1016/j.foodchem.2016.11.035. Epub 2016 Nov 9.
In this study, a magnetic graphene-based dispersive solid phase extraction method was developed that was combined with high performance liquid chromatography to determine the residues of fluoroquinolone drugs in foods of animal origin. During the experiments, several parameters possible influencing the extraction performance were optimized (amount of magnetic graphene, sample pH, extraction time and elution solution). This extraction method showed high absorption capacities (>6800ng) and high enrichment factors (68-79-fold) for seven fluoroquinolones. Furthermore, this absorbent could be reused for at least 40 times. The limits of detection were in the range of 0.05-0.3ng/g, and the recoveries from the standards fortified blank samples (bovine milk, chicken muscle and egg) were in the range of 82.4-108.5%. Therefore, this method could be used as a simple and sensitive tool to determine the residues of fluoroquinolones in foods of animal origin.
在本研究中，开发了一种基于磁性石墨烯的分散固相萃取方法，并将其与高效液相色谱联用，用于测定动物源性食品中氟喹诺酮类药物的残留量。在实验过程中，对几个可能影响萃取性能的参数（磁性石墨烯用量、样品pH值、萃取时间和洗脱溶液）进行了优化。该萃取方法对七种氟喹诺酮类药物显示出高吸附容量（>6800ng）和高富集因子（68-79倍）。此外，这种吸附剂至少可重复使用40次。检测限在0.05-0.3ng/g范围内，加标空白样品（牛奶、鸡肉和鸡蛋）的回收率在82.4-108.5%范围内。因此，该方法可作为一种简单灵敏的工具，用于测定动物源性食品中氟喹诺酮类药物的残留量。
