Université de Tunis El Manar, Faculty of Medicine of Tunis, Department of Basic Sciences, Tunis, Tunisia.
Université de Tunis El Manar, Faculty of Medicine of Tunis, Department of Basic Sciences, Tunis, Tunisia; A. Mami Hospital, Department of Pediatric Respiratory Diseases, Unit Research 12SP15 "Expression Moleculaire des Interactions Cellulaires et leur Mode d'Action dans le Poumon Profond", Pavillon B, 2080, Ariana, Tunisia.
Cytokine. 2018 Apr;104:85-91. doi: 10.1016/j.cyto.2017.09.028. Epub 2017 Oct 3.
Interleukin-33 (IL-33) is one of the last discovered members of the human IL-1 family. It is involved in the pathogenesis of many inflammatory diseases. This study investigates the relationship between IL33 gene variants and serum protein levels with the development of childhood asthma. We analyzed in this case-control study the distribution of two IL33 polymorphisms, rs7044343 and rs1342326, within 200 Tunisian children, using predefined Taqman genotyping assays. IL-33 serum levels were assessed by commercial sandwich Enzyme-linked immunosorbent assay (ELISA). The presence of rs1342326 polymorphism was significantly associated with a lower risk of asthma development. The CC [OR=0.20, CI (0.08-0.50)] and AC [OR=0.24, CI (0.11-0.49)] genotypes, as well as the C-allele [OR=0.40; CI: 0.26-0.61, P=0.00001] were associated significantly with a decreased asthma risk. However, the C-allele was more frequent in severe asthma patients than in milder ones. No association was found between rs7044343 variant and asthma. The level of IL-33 in sera was significantly increased in asthmatic children [1.48±0.47pg/mL] compared to controls [0.70±0.18pg/mL; P<0.001]. Furthermore, this increase of IL-33 was associated with the presence of rs1342326 C allele. The IL33 rs1342326 polymorphism was associated with a lower childhood asthma risk in the Tunisian population and a higher IL-33 protein expression.
白细胞介素-33 (IL-33) 是人类白细胞介素-1 家族中最后发现的成员之一。它参与了许多炎症性疾病的发病机制。本研究探讨了 IL33 基因变异与血清蛋白水平与儿童哮喘发展之间的关系。我们在这项病例对照研究中，使用预定义的 Taqman 基因分型测定法，分析了 200 名突尼斯儿童中两个 IL33 多态性 rs7044343 和 rs1342326 的分布情况。通过商业夹心酶联免疫吸附测定法 (ELISA) 评估 IL-33 血清水平。rs1342326 多态性的存在与哮喘发展的风险降低显著相关。CC[OR=0.20,CI(0.08-0.50)]和 AC[OR=0.24,CI(0.11-0.49)]基因型以及 C 等位基因[OR=0.40;CI:0.26-0.61,P=0.00001]与哮喘风险降低显著相关。然而，C 等位基因在重症哮喘患者中比在轻症患者中更为常见。rs7044343 变异与哮喘之间未发现关联。哮喘患儿血清中 IL-33 水平显著升高[1.48±0.47pg/mL]，对照组为[0.70±0.18pg/mL；P<0.001]。此外，IL-33 的这种增加与 rs1342326 C 等位基因的存在相关。IL33 rs1342326 多态性与突尼斯人群中儿童哮喘风险降低和更高的 IL-33 蛋白表达相关。
