University of Tunis El Manar, Faculty of Medicine of Tunis, Department of Basic Sciences, Tunis, Tunisia; Abderrahman Mami Hospital, Department of Pediatric Respiratory Diseases, Unit Research 19SP02 "Exploration of the Deep Lung: From Gene to Management", Pavilion B, 2080 Ariana, Tunisia.
Abderrahman Mami Hospital, Department of Pediatric Respiratory Diseases, Unit Research 19SP02 "Exploration of the Deep Lung: From Gene to Management", Pavilion B, 2080 Ariana, Tunisia.
Cytokine. 2020 Oct;134:155206. doi: 10.1016/j.cyto.2020.155206. Epub 2020 Jul 16.
The interleukin-26 (IL-26), a member of the IL-10 family is one of the latest discovered cytokines which contributes in numerous chronic autoimmune and inflammatory disorders. In the current case-control study, we investigated the distribution of three IL-26 single nucleotide polymorphisms (SNPs) (rs7134599, rs2870946 & rs1558744) in 440 Tunisian adults via Taqman genotyping assay. The presence of rs7134599 and rs1558744 polymorphisms considerably reduced the risk of developing asthma while the rs7134599 AA [OR = 0.40, CI: 0.23-0.70] and AG [OR = 0.50, CI (0.32-0.76)] genotypes protected against the asthma risk. The rs7134599 A allele was correlated with a lower risk of developing severe asthma (p < 0.001) while that of the rs2870946 CC genotype was associated with a higher risk of developing asthma in smoking patients (p < 0.001). In addition, we measured the IL-26 levels in the serum by an Enzyme-linked-Immunosorbent Assay (ELISA). During the analysis, we found that IL-26 serum levels were incredibly increased in asthmatic patients compared to the healthy controls. Our study revealed a significant association of IL-26 gene polymorphisms with asthma for the first time which can serve as biomarkers for asthma in the Tunisian population. The significant increase of IL-26 serum protein levels in asthma patients suggested a major role of IL-26 in asthma phenotypes.
白细胞介素-26（IL-26）是 IL-10 家族的一员，是最新发现的细胞因子之一，它参与了许多慢性自身免疫和炎症性疾病。在目前的病例对照研究中，我们通过 Taqman 基因分型检测法调查了 440 名突尼斯成年人中三个 IL-26 单核苷酸多态性（SNP）（rs7134599、rs2870946 和 rs1558744）的分布。rs7134599 和 rs1558744 多态性的存在显著降低了哮喘发病风险，而 rs7134599 AA [OR=0.40,CI:0.23-0.70]和 AG [OR=0.50,CI(0.32-0.76)]基因型则能预防哮喘风险。rs7134599 A 等位基因与发生严重哮喘的风险降低相关（p<0.001），而 rs2870946 CC 基因型则与吸烟患者发生哮喘的风险升高相关（p<0.001）。此外，我们还通过酶联免疫吸附测定（ELISA）法测量了血清中的 IL-26 水平。在分析过程中，我们发现哮喘患者的血清 IL-26 水平显著高于健康对照组。我们的研究首次揭示了 IL-26 基因多态性与哮喘之间存在显著关联，这可能成为突尼斯人群哮喘的生物标志物。哮喘患者的血清 IL-26 蛋白水平显著升高，提示 IL-26 在哮喘表型中起着重要作用。
