Department of Chemical Engineering & Technology, Indian Institute of Technology (BHU) Varanasi, Varanasi, 221005, India.
Appl Biochem Biotechnol. 2018 Apr;184(4):1247-1262. doi: 10.1007/s12010-017-2620-3. Epub 2017 Oct 6.
Growths of Lyngbya limnetica and Oscillatoria obscura were investigated at varying pH, light intensity, temperature, and trace element concentration with a view to optimize these parameters for obtaining the maximum carbohydrate content. The maximum growth for both strains was obtained at pH 9.0 and temperature 20 ± 3 °C using a light intensity of 68.0 μmol m s with continuous shaking. Growth under the nitrogen starvation condition affected the carbohydrate content more compared to the phosphorus starvation, and maximum concentrations were found as 0.660 and 0.621 g/g of dry biomass for L. limnetica and O. obscura, respectively. Under the optimized nitrogen-rich conditions, the specific growth rates for the two strains were found to be 0.187 and 0.215 day, respectively. The two-stage growth studies under nitrogen-rich (stage I) followed by nitrogen starvation (stage II) conditions were performed, and maximum biomass and carbohydrate productivity were found as 0.088 and 0.423 g L day for L. limnetica. This is the first ever attempt to evaluate and optimize various parameters affecting the growth of cyanobacterial biomass of L. limnetica and O. obscura as well as their carbohydrate contents.
研究了 Lyngbya limnetica 和 Oscillatoria obscura 在不同 pH 值、光照强度、温度和微量元素浓度下的生长情况，以期优化这些参数，以获得最大的碳水化合物含量。两种菌株的最大生长均在 pH 值为 9.0 和温度 20 ± 3°C 下获得，使用 68.0 μmol m s 的光照强度并进行连续搅拌。与磷饥饿相比，氮饥饿条件下的生长对碳水化合物含量的影响更大，最大浓度分别为 L. limnetica 和 O. obscura 的 0.660 和 0.621 g/g 干生物质。在优化的富氮条件下，两种菌株的比生长速率分别为 0.187 和 0.215 天。进行了富氮（阶段 I）随后氮饥饿（阶段 II）条件下的两阶段生长研究，发现 L. limnetica 的最大生物量和碳水化合物生产力分别为 0.088 和 0.423 g L day。这是首次评估和优化影响 Lyngbya limnetica 和 Oscillatoria obscura 蓝藻生物质生长及其碳水化合物含量的各种参数的尝试。
