体外致瘤性检测：癌症干细胞的集落形成检测
In Vitro Tumorigenic Assay: Colony Forming Assay for Cancer Stem Cells.
作者信息
Rajendran Vijayalakshmi, Jain Mayur Vilas
机构信息
Section of Immunity, Infection and Inflammation, School of Medicine, Medical Sciences and Nutrition, Institute of Medical Sciences, University of Aberdeen, Scotland, AB25 2ZD, UK.
Division of Oto-Rhino-Laryngology, Head and Neck Surgery, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Linköping, SE-581 85, Sweden.
出版信息
Methods Mol Biol. 2018;1692:89-95. doi: 10.1007/978-1-4939-7401-6_8.
Colony forming or clonogenic assay is an in vitro quantitative technique to examine the capability of a single cell to grow into a large colony through clonal expansion. Clonogenic activity is a sensitive indicator of undifferentiated cancer stem cells. Here, we described the colony forming ability of the isolated breast cancer stem cells from the total population of cancer cells using double-layered, soft agarose-based assay. This method demonstrates that cancer stem cells can survive and generate colony growth in an anchorage-independent culture model. The 0.005% crystal violet solution is used in this assay to visualize the generated colonies.
集落形成或克隆形成试验是一种体外定量技术，用于检测单个细胞通过克隆扩增生长成大菌落的能力。克隆形成活性是未分化癌干细胞的敏感指标。在此，我们使用基于双层软琼脂糖的试验描述了从癌细胞总体中分离出的乳腺癌干细胞的集落形成能力。该方法表明，癌干细胞可以在非锚定依赖的培养模型中存活并产生集落生长。此试验中使用0.005%的结晶紫溶液来观察所形成的菌落。
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