De Angelis Maria Laura, De Maria Ruggero, Baiocchi Marta
Department of Oncology and Molecular Medicine, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Rome, Italy.
Department of General Pathology, Università Cattolica del Sacro Cuore, Rome, Italy.
Methods Mol Biol. 2018;1692:107-115. doi: 10.1007/978-1-4939-7401-6_10.
Banks of genetically characterized cancer stem cells (CSCs) isolated from individual patients and grown as spheroids offer an invaluable approach to identify genetic determinants of drug resistance versus sensitivity, and to study new stem cell-directed therapies. Here, we describe our standardized procedure for in vitro drug screening on colorectal CSCs, taking irinotecan as an example.
从个体患者中分离并培养成球状体的基因特征明确的癌症干细胞（CSCs）库，为确定耐药性与敏感性的基因决定因素以及研究新的干细胞导向疗法提供了一种非常有价值的方法。在此，我们以伊立替康为例，描述我们针对结直肠癌CSCs进行体外药物筛选的标准化程序。
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