Department of Dermatology, Leiden University Medical Center, Leiden, The Netherlands.
Department of Medical Statistics and Bioinformatics, Leiden University Medical Center, Leiden, The Netherlands.
Exp Dermatol. 2018 Oct;27(10):1172-1175. doi: 10.1111/exd.13453. Epub 2017 Dec 20.
We studied Lgr6 stem cells in experimental UV carcinogenesis in hairless mice. For further characterization through RNA-seq, these stem cells were isolated by FACS from transgenic hairless mice bearing an EGFP-Ires-CreERT2 reporter cassette inserted into exon 1 of the Lgr6 gene (purity confirmed by human ERT2 expression). Between Lgr6/EGFP and Lgr6/EGFP basal cells (Tg/wt), 682 RNAs were differentially expressed, indicating stemness and expression of cancer-related pathways in Lgr6/EGFP cells. We discovered that suspected "Lgr6 null" mice (Tg/Tg) expressed RNA of an Lgr6 isoform (delta-Lgr6, lacking 74 N-terminal aa) which could be functional and explain the lack of a phenotype.
我们在无毛小鼠的实验性 UV 致癌作用中研究了 Lgr6 干细胞。为了通过 RNA-seq 进行进一步表征，这些干细胞通过 FACS 从携带 EGFP-Ires-CreERT2 报告基因盒插入 Lgr6 基因外显子 1 的转基因无毛小鼠中分离出来（通过人 ERT2 表达确认纯度）。在 Lgr6/EGFP 和 Lgr6/EGFP 基础细胞（Tg/wt）之间，有 682 个 RNA 表达差异，表明 Lgr6/EGFP 细胞具有干性和表达与癌症相关的途径。我们发现，疑似“Lgr6 缺失”小鼠（Tg/Tg）表达了一种 Lgr6 同工型（delta-Lgr6，缺少 74 个 N 端 aa）的 RNA，该 RNA 可能具有功能并解释缺乏表型的原因。
