Lee Seung-Chul, Lee Chang-Lyoul, Heo Jeongyun, Jeong Chan-Uk, Lee Gyeong-Hui, Kim Sehoon, Yoon Woojin, Yun Hoseop, Park Sung O, Kwak Sang Kyu, Park Sung-Ha, Kwon O-Pil
Department of Molecular Science and Technology, Ajou University, Suwon, 443-749, Republic of Korea.
Advanced Photonics Research Institute (APRI), Gwangju Institute of Science and Technology (GIST), 123 Cheomdangwagi-ro, Buk-gu, Gwangju, 61005, Republic of Korea.
Chemistry. 2018 Feb 26;24(12):2888-2897. doi: 10.1002/chem.201704036. Epub 2017 Dec 4.
A series of fluorescent molecular rotors obtained by introducing two rotational groups ("rotators"), which exhibit different rotational and electron-donating abilities, are discussed. Whereas the control molecular rotor, PH, includes a single rotator (the widely used phenyl group), the PO molecular rotors consist of two rotators (a phenyl group and an alkoxy group), which exhibit simultaneous strongly electron-donating and easy rotational abilities. Compared with the control rotor PH, PO molecular rotors exhibited one order of magnitude higher quantum yield (fluorescence intensity) and simultaneously exhibited significantly higher fluorescence contrast. These properties are directly related to the strong electron-donating ability and low energy barrier of rotation of the alkoxy group, as confirmed by dynamic fluorescence experiments and quantum chemical calculations. The PO molecular rotors exhibited two fluorescence relaxation pathways, whereas the PH molecular rotor exhibited a single fluorescence relaxation pathway. Cellular fluorescence imaging with PO molecular rotors for mapping cellular viscosity was successfully demonstrated.
本文讨论了一系列通过引入两个具有不同旋转和给电子能力的旋转基团（“转子”）而获得的荧光分子转子。对照分子转子PH包含一个单一的转子（广泛使用的苯基），而PO分子转子由两个转子（一个苯基和一个烷氧基）组成，它们同时具有强给电子能力和易于旋转的能力。与对照转子PH相比，PO分子转子的量子产率（荧光强度）高一个数量级，同时荧光对比度也显著更高。动态荧光实验和量子化学计算证实，这些性质与烷氧基的强给电子能力和低旋转能垒直接相关。PO分子转子表现出两种荧光弛豫途径，而PH分子转子表现出单一的荧光弛豫途径。成功地展示了使用PO分子转子进行细胞荧光成像以绘制细胞粘度图。
