Olszewska E, Hay D E, Jones K W, Chetty U
Department of Genetics, Edinburgh University, UK.
Oncogene. 1987;1(4):403-8.
Bkm-related probe 2[8], consisting mainly of GATA repeats, detects a hypervariable pattern of restriction fragment length polymorphisms in human DNA which is normally developmentally stable. However, specific somatic DNA variability was found in 53% (7/13) of human breast carcinomas, but not in eight cases of bladder carcinoma, when compared with leukocyte DNAs from the same patient under the same conditions. Certain of the restriction fragments detected carry both GATA-related sequences and sequences related to the M13 vector. Comparison of BstN1 digests of tumour and cognate leukocyte DNA revealed a further variable ethidium-stained restriction fragment class, not recognised by the probe, in 93% of breast and in 63% of bladder carcinomas.
主要由GATA重复序列组成的Bkm相关探针2[8]，可检测人类DNA中限制性片段长度多态性的高变模式，该模式在正常发育过程中是稳定的。然而，在相同条件下，与同一患者的白细胞DNA相比，在53%（7/13）的人类乳腺癌中发现了特定的体细胞DNA变异性，但在8例膀胱癌中未发现。所检测到的某些限制性片段同时携带与GATA相关的序列和与M13载体相关的序列。肿瘤和同源白细胞DNA的BstN1酶切产物比较显示，在93%的乳腺癌和63%的膀胱癌中，存在一类未被该探针识别的、经溴化乙锭染色的可变限制性片段。
