增强产聚羟基烷酸的生产及特性研究由 Natrinema ajinwuensis （同义名） ≡ Natrinema altunense 菌株 RM-G10 产生。
Production enhancement and characterization of the polyhydroxyalkanoate produced by Natrinema ajinwuensis (as synonym) ≡ Natrinema altunense strain RM-G10.
机构信息
School of Environmental Studies, Jadavpur University, Kolkata 700 032, India.
Department of Chemistry, Jadavpur University, Kolkata 700 032, India.
出版信息
Int J Biol Macromol. 2018 Feb;107(Pt B):1480-1490. doi: 10.1016/j.ijbiomac.2017.10.009. Epub 2017 Oct 5.
Application of halophiles can decrease the cost of polyhydroxyalkanoate (PHA) production or bioplastic which are an alternative to the petroleum-derived plastic. Extremely halophilic archaeon, Natrinema ajinwuensis RM-G10 accumulated 61.02±0.68% PHA of its cell dry mass at 72h in repeated batch cultures yielding 0.210±0.001gLh volumetric productivity after selection of the best cultivation conditions. Transmission electron microscopy showed the presence of PHA granules inside the archaeal cells. Characterization by gas chromatographic analysis, gas chromatographic- mass spectrophotometric analysis, thermogravimetric analysis, differential scanning calorimetric analysis, X-ray diffraction analysis, Fourier transform infra red spectroscopy and nuclear magnetic resonance spectroscopy revealed the polymer to be poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with 13.93mol% 3-hydroxyvalerate content and having 35.45% crystallinity, -12.3°C glass transition temperature, 143°C and 157.5°C melting temperatures and 284°C degradation temperature. This is the first report on production enhancement (on a small scale) and characterization of the polyhydroxyalkanoate produced by Natrinema ajinwuensis (as synonym) ≡ Natrinema altunense strain RM-G10 and the Natrinema genus in general.
嗜盐菌的应用可以降低聚羟基烷酸酯（PHA）或生物塑料的生产成本，这些物质是石油衍生塑料的替代品。极端嗜盐古菌 Natrinema ajinwuensis RM-G10 在重复分批培养中，在最佳培养条件下选择后，细胞干重的 61.02±0.68%聚积为 PHA，体积生产率为 0.210±0.001gLh。透射电子显微镜显示古菌细胞内存在 PHA 颗粒。通过气相色谱分析、气相色谱-质谱分光光度分析、热重分析、差示扫描量热分析、X 射线衍射分析、傅里叶变换红外光谱和核磁共振光谱进行的特性分析表明，该聚合物为聚（3-羟基丁酸酯-co-3-羟基戊酸酯），其中 3-羟基戊酸含量为 13.93mol%，结晶度为 35.45%，玻璃化转变温度为-12.3°C，熔融温度为 143°C 和 157.5°C，降解温度为 284°C。这是关于 Natrinema ajinwuensis（同义）≡Natrinema altunense 菌株 RM-G10 以及 Natrinema 属生产的聚羟基烷酸酯（PHA）的生产增强（小规模）和特性的首次报告。
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