State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
Plant Physiol Biochem. 2017 Nov;120:61-74. doi: 10.1016/j.plaphy.2017.09.020. Epub 2017 Sep 28.
NAC transcription factors (TFs) belong to plant-specific TFs, which have been identified in many plant species. The NAC TFs act as the nodes of a regulatory network in plant's response to abiotic and biotic stresses. Till now, response of tomato NAC TFs involved in Tomato yellow leaf curl virus (TYLCV) infection is unknown. In the present study, six NAC TFs were identified to respond to TYLCV infection in tomato. We observed that transcripts of four NAC genes (SlNAC20, SlNAC24, SlNAC47, and SlNAC61) were induced after TYLCV infection in resistant tomato cultivar. Virus-induced gene silencing analysis (VIGS) indicated that SlNAC61 played positive roles in response to TYLCV infection. Tomato NAC TFs were not only involved in defense regulation but in development and stress progress. These NAC TFs interacted with other proteins, including protein phosphatase and mitogen-activated protein kinase. Some defense response TFs, such as WRKY, TGA, MYB, NAC, could interact with NAC proteins by binding cis-elements in promoter regions of NAC TFs. These identified tomato NAC TFs cooperated with other TFs and proteins, indicating the complex response mechanism of described NAC TFs involved in TYLCV infection. The results will offer new evidence to further understand the NAC TFs involved in response to TYLCV infection in tomato.
NAC 转录因子（TFs）属于植物特异性 TFs，已在许多植物物种中被鉴定出来。NAC TFs 作为植物应对非生物和生物胁迫反应的调控网络的节点。迄今为止，参与番茄黄曲叶病毒（TYLCV）感染的番茄 NAC TFs 的反应尚不清楚。在本研究中，鉴定了 6 个参与番茄 TYLCV 感染的 NAC TFs。我们观察到，在抗 TYLCV 番茄品种中，4 个 NAC 基因（SlNAC20、SlNAC24、SlNAC47 和 SlNAC61）的转录本在 TYLCV 感染后被诱导。病毒诱导的基因沉默分析（VIGS）表明 SlNAC61 在响应 TYLCV 感染中发挥积极作用。番茄 NAC TFs 不仅参与防御调控，还参与发育和应激进程。这些 NAC TFs 与其他蛋白质相互作用，包括蛋白磷酸酶和丝裂原活化蛋白激酶。一些防御反应 TFs，如 WRKY、TGA、MYB、NAC，可以通过结合 NAC TFs 启动子区域中的顺式元件与 NAC 蛋白相互作用。这些鉴定出的番茄 NAC TFs 与其他 TFs 和蛋白质合作，表明所描述的 NAC TFs 参与 TYLCV 感染的复杂反应机制。研究结果将为进一步了解参与番茄 TYLCV 感染反应的 NAC TFs 提供新的证据。
