Bumann Dirk, Cunrath Olivier
Focal Area Infection Biology, Biozentrum, University of Basel, Klingelbergstr. 50/70, CH-4056 Basel, Switzerland.
Focal Area Infection Biology, Biozentrum, University of Basel, Klingelbergstr. 50/70, CH-4056 Basel, Switzerland.
Curr Opin Microbiol. 2017 Oct;39:57-63. doi: 10.1016/j.mib.2017.09.008. Epub 2017 Oct 6.
Infected host tissues have complex anatomy, diverse cell types, and dynamic inflammation. Traditional infection biology approaches largely ignore this complex host environment and its impact on pathogens, but recent single-cell technologies unravel extensively heterogeneous host-pathogen interactions in vivo. Salmonella are major model pathogens in this field due to the availability of excellent mouse disease models and facile molecular biology. The results show how Salmonella stochastically vary their virulence, exploit differential nutrient availability, experience and respond to widely varying stresses, and have disparate fates ranging from vigorous proliferation to eradication within the same host tissue. Specific Salmonella subsets drive disease progression, while others persist during antimicrobial chemotherapy. Further elucidation of the underlying mechanisms could provide a basis for improved infection control.
受感染的宿主组织具有复杂的解剖结构、多样的细胞类型和动态的炎症反应。传统的感染生物学方法在很大程度上忽略了这种复杂的宿主环境及其对病原体的影响，但最近的单细胞技术揭示了体内广泛存在的异质性宿主-病原体相互作用。由于有出色的小鼠疾病模型和简便的分子生物学方法，沙门氏菌是该领域主要的模式病原体。结果表明，沙门氏菌如何随机改变其毒力，利用不同的营养可利用性，经历并应对广泛变化的压力，以及在同一宿主组织内具有从旺盛增殖到被根除的不同命运。特定的沙门氏菌亚群推动疾病进展，而其他亚群在抗菌化疗期间持续存在。进一步阐明其潜在机制可为改善感染控制提供依据。
