Wang Zhen, Liu Hongxu, Liu Jiaxiu, Ren Xiaomeng, Song Guoku, Xia Xiaodong, Qin Ningbo
Collaborative Innovation Center of Seafood Deep Processing, National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116304, China.
Sino-US Joint Research Center for Food Safety, College of Food Science and Engineering, Northwest A & F University, Xianyang 712100, China.
Foods. 2021 Dec 3;10(12):2990. doi: 10.3390/foods10122990.
Acrylamide (AA) has been extensively examined for its potential toxicological effects on humans and animals, but its impacts on gut microbiota and effects on hosts' susceptibility to enteric infection remain elusive. The present study was designed to evaluate the effect of AA on gut microbiota of mice and susceptibility of mice to . Typhimurium infection. After four weeks' intervention, mice fed with AA exhibited significantly decreased body weight. Meanwhile, 16S rRNA gene sequencing showed reduced relative abundance of Firmicutes and increased abundance of Bacteroidetes in AA-treated mice prior to infection. In addition, we observed high relative abundance of and , more specifically the genus and , respectively, in AA-treated mice before infection. Subsequently, the mice were orally infected with . Typhimurium. The histological changes, systemic dissemination of . Typhimurium, and inflammatory responses were examined. Compared to mice fed with normal diet, mice fed AA exhibited higher level of bacterial counts in liver, spleen, and ileum, which was consistent with exacerbated tissue damage determined by histological analyses. In addition, higher expression of pro-inflammaroty cytokines, p-IκBα, and p-P65 and lower mRNA expressions of , , , , and were detected in AA-treated mice. These findings provide novel insights into the potential health impact of AA consumption and the detailed mechanism for its effect on . Typhimurium infection merit further exploration.
丙烯酰胺（AA）已被广泛研究其对人和动物的潜在毒理学影响，但其对肠道微生物群的影响以及对宿主肠道感染易感性的作用仍不清楚。本研究旨在评估AA对小鼠肠道微生物群的影响以及小鼠对鼠伤寒沙门氏菌感染的易感性。经过四周的干预，喂食AA的小鼠体重显著下降。同时，16S rRNA基因测序显示，在感染前，经AA处理的小鼠中厚壁菌门的相对丰度降低，拟杆菌门的丰度增加。此外，我们在感染前经AA处理的小鼠中分别观察到了[具体菌属1]和[具体菌属2]的相对丰度较高，更具体地说是[具体菌属1]属和[具体菌属2]属。随后，小鼠经口感染鼠伤寒沙门氏菌。检查了组织学变化、鼠伤寒沙门氏菌的全身扩散和炎症反应。与喂食正常饮食的小鼠相比，喂食AA的小鼠在肝脏、脾脏和回肠中的细菌计数水平更高，这与组织学分析确定的组织损伤加剧一致。此外，在经AA处理的小鼠中检测到促炎细胞因子、p-IκBα和p-P65的表达较高，而[相关基因1]、[相关基因2]、[相关基因3]、[相关基因4]和[相关基因5]的mRNA表达较低。这些发现为食用AA的潜在健康影响提供了新的见解，其对鼠伤寒沙门氏菌感染影响的详细机制值得进一步探索。
