Xu Jian-Hui, Hou Xiao-Yu, Tang Yu, Luo Rong, Zhang Jie, Liu Chang, Yang Yong-Lu
Thermoregulation and Inflammation Laboratory, Chengdu Medical College, Chengdu, Sichuan 610500, China.
Department of Obstetrics, The First Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan 610500, China.
Neurosci Lett. 2018 Jan 1;662:59-64. doi: 10.1016/j.neulet.2017.10.002. Epub 2017 Oct 6.
Arginine vasopressin (AVP) plays an important role in thermoregulation and antipyresis. We have demonstrated that AVP could change the spontaneous activity of thermosensitive and temperature insensitive neurons in the preoptic area. However, whether AVP influences the effects of prostaglandin E (PGE) on the spontaneous activity of neurons in the medial preoptic area (MPO) remains unclear. Our experiment showed that PGE decreased the spontaneous activity of warm-sensitive neurons, and increased that of low-slope temperature-insensitive neurons in the MPO. AVP attenuated the inhibitory effect of PGE on warm-sensitive neurons, and reversed the excitatory effect of PGE on low-slope temperature-insensitive neurons, demonstrating that AVP antagonized the effects of PGE on the spontaneous activity of these neurons. The effect of AVP was suppressed by an AVP V receptor antagonist, suggesting that V receptor mediated the action of AVP. We also demonstrated that AVP attenuated the PGE-induced decrease in the prepotential's rate of rise in warm-sensitive neurons and the PGE-induced increase in that in low-slope temperature-insensitive neurons through the V receptor. Together, these data indicated that AVP antagonized the PGE-induced change in the spontaneous activity of warm-sensitive and low-slope temperature-insensitive neurons in the MPO partly by reducing the PGE-induced change in the prepotential of these neurons in a V receptor-dependent manner.
精氨酸加压素（AVP）在体温调节和退热中起重要作用。我们已经证明，AVP可以改变视前区热敏和温度不敏感神经元的自发活动。然而，AVP是否影响前列腺素E（PGE）对视前内侧区（MPO）神经元自发活动的作用仍不清楚。我们的实验表明，PGE降低了MPO中温敏神经元的自发活动，并增加了低斜率温度不敏感神经元的自发活动。AVP减弱了PGE对温敏神经元的抑制作用，并逆转了PGE对低斜率温度不敏感神经元的兴奋作用，表明AVP拮抗了PGE对这些神经元自发活动的影响。AVP V受体拮抗剂抑制了AVP的作用，提示V受体介导了AVP的作用。我们还证明，AVP通过V受体减弱了PGE诱导的温敏神经元预电位上升速率的降低以及PGE诱导的低斜率温度不敏感神经元预电位上升速率的增加。总之，这些数据表明，AVP部分通过以V受体依赖的方式减少PGE诱导的这些神经元预电位的变化，拮抗了PGE诱导的MPO中温敏和低斜率温度不敏感神经元自发活动的变化。
