Pal Hilal Ahmad, Mohapatra Saswat, Gupta Varsha, Ghosh Surajit, Verma Sandeep
Department of Chemistry and Center for Environmental Science and Engineering , Indian Institute of Technology Kanpur , Kanpur 208016 , UP , India . Email:
Department of Organic and Medicinal Chemistry , CSIR-Indian Institute of Chemical Biology Kolkata , 4, Raja S. C. Mullick Road , Jadavpur 700032 , WB , India.
Chem Sci. 2017 Sep 1;8(9):6171-6175. doi: 10.1039/c6sc05017d. Epub 2017 Jun 20.
Nitric oxide (NO), an endogenously produced free radical species, is an extremely important signalling molecule in several biochemical processes related to neurotransmission, neuronal communication, and vasodilation, to name a few. Other than relying on endogenous synthesis, intracellular NO delivery presents an interesting challenge to fully exploit the therapeutic potential of this gaseous molecule. We have applied a self-assembling peptide conjugate strategy to devise a construct carrying a NO-release arm, which can be activated under standard redox conditions. Consequently, a tryptophan-based peptide carrier was designed, which self-assembled in the solution phase to afford soft nanospherical structures, and released NO in Neuro2a cell line, resulting in neurite outgrowth.
一氧化氮（NO）是一种内源性产生的自由基，在与神经传递、神经元通讯和血管舒张等多种生化过程中，是一种极其重要的信号分子。除了依赖内源性合成外，细胞内一氧化氮的递送对于充分发挥这种气态分子的治疗潜力提出了一个有趣的挑战。我们应用了一种自组装肽共轭策略来设计一种带有一氧化氮释放臂的构建体，该构建体可以在标准氧化还原条件下被激活。因此，设计了一种基于色氨酸的肽载体，它在溶液相中自组装形成柔软的纳米球结构，并在Neuro2a细胞系中释放一氧化氮，从而导致神经突生长。
