Department of Chemistry, University of British Columbia, Vancouver, BC, Canada.
Electrophoresis. 2018 May;39(9-10):1216-1221. doi: 10.1002/elps.201700364. Epub 2017 Oct 31.
A nonaqueous capillary electrophoresis mass spectrometry (NACE-MS) method was developed to separate and determine highly hydrophobic temporin peptides. The nonaqueous background electrolyte solution was a mixture of 20% acetonitrile, 78% methanol and 2% formic acid, with 20 mM ammonium formate. The separation of six peptides was completed within 12 min. The CE system was connected to a triple quadrupole mass spectrometer operating in MRM mode using a chemical modifier solution of 2 mM ammonium formate in ethanol with the flow through microvial interface. The mass spectrometer offered a second dimension of separation for peptides having identical migration times but different structures. The new method represents the first system capable of reliably determining hydrophobic peptides without using reversed phase liquid chromatography mass spectrometry.
建立了一种非水毛细管电泳-质谱（NACE-MS）方法，用于分离和测定高度疏水性的防御素肽。非水背景电解质溶液为 20%乙腈、78%甲醇和 2%甲酸的混合物，其中含有 20 mM 甲酸铵。六种肽在 12 分钟内完成分离。CE 系统与三重四极杆质谱仪连接，采用乙醇中 2 mM 甲酸铵的化学改性剂溶液在微流路接口的方式进行 MRM 模式操作。质谱仪为具有相同迁移时间但不同结构的肽提供了二维分离。该新方法代表了第一个无需使用反相液相色谱-质谱法即可可靠测定疏水性肽的系统。
