循环微小RNA-27a/b在急性肺栓塞患者中的诊断价值

Diagnostic value of circulating microRNA-27a/b in patients with acute pulmonary embolism.

作者信息

Wang Qian, Ma Junfen, Jiang Zhiyun, Wu Fan, Ping Jiedan, Ming Liang

机构信息

Department of Clinical Laboratory, the First Affiliated Hospital of Zhengzhou University, Key Laboratory of Laboratory Medicine of Henan Province, Zhengzhou, Henan, China.

Department of Clinical Laboratory, the First Affiliated Hospital of Zhengzhou University, Key Laboratory of Laboratory Medicine of Henan Province, Zhengzhou, Henan, China -

出版信息

Int Angiol. 2018 Feb;37(1):19-25. doi: 10.23736/S0392-9590.17.03877-9. Epub 2017 Oct 9.

DOI:10.23736/S0392-9590.17.03877-9

PMID:28990745

Abstract

BACKGROUND

Circulating microRNAs (miRNAs) have been increasingly suggested as biomarkers for numerous diseases. The aims of this study were to evaluate the expression of plasma miR-27a/b in patients with acute pulmonary embolism (APE) and determine the possibility of miR-27a/b as diagnostic biomarkers for APE.

METHODS

Seventy-eight APE patients diagnosed by computed tomographic pulmonary angiography (CTPA) and 70 age and gender matched normal volunteers were included in this study. The levels of miR-27a and miR-27b were measured by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and the concentrations of plasma D-dimer were measured using immunoturbidimetric assay.

RESULTS

The levels of plasma miR-27a and miR-27b were significantly higher in APE patients (P<0.001) compared with normal controls. Receiver operating characteristic (ROC) curve analyses showed that plasma miR-27a was superior to miR-27b for the diagnosis of APE (AUC=0.784, AUC=0.707, respectively). Combining miR-27a or miR-27b with D-dimer significantly increased the diagnostic capacity of APE.

CONCLUSIONS

Our results showed that circulating miR-27a and miR-27b might be potential novel diagnostic biomarkers in APE patients.

摘要

背景

循环微小RNA（miRNA）越来越多地被认为是多种疾病的生物标志物。本研究旨在评估急性肺栓塞（APE）患者血浆miR-27a/b的表达，并确定miR-27a/b作为APE诊断生物标志物的可能性。

方法

本研究纳入了78例经计算机断层扫描肺动脉造影（CTPA）诊断的APE患者和70例年龄及性别匹配的正常志愿者。采用定量逆转录-聚合酶链反应（qRT-PCR）检测miR-27a和miR-27b的水平，采用免疫比浊法检测血浆D-二聚体的浓度。

结果

与正常对照组相比，APE患者血浆miR-27a和miR-27b水平显著升高（P<0.001）。受试者工作特征（ROC）曲线分析表明，血浆miR-27a在诊断APE方面优于miR-27b（AUC分别为0.784和0.707）。将miR-27a或miR-27b与D-二聚体联合使用可显著提高APE的诊断能力。