Department of Respiratory Medicine, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning, China (mainland).
Med Sci Monit. 2018 Nov 28;24:8621-8626. doi: 10.12659/MSM.910893.
BACKGROUND The aim of this study was to measure and compare the plasma levels of the microRNA (miRNA), miR-221, in patients with acute pulmonary embolism (PE) with healthy individuals and to evaluate the potential role of miR-221 as a diagnostic biomarker for acute PE. MATERIAL AND METHODS In blood samples collected from 60 patients with acute PE and 50 healthy volunteers, plasma levels of microRNA were identified using a microRNA microarray, and miR-221 expression was detected using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). Brain natriuretic peptide (BNP) and troponin I were measured using an automated immunoassay analyzer. D-dimer levels were measured with an enzyme-linked immunosorbent assay (ELISA). RESULTS From the evaluation of 32 differentially expressed plasma miRNAs, miR-221 was significantly upregulated in the plasma of patients with acute PE compared with normal individuals (P<0.05). Correlation analysis showed that plasma miR-221 levels in patients with acute PE were positively correlated with levels of BNP (r=0.842, P<0.05), troponin I (r=0.853; P<0.05), and D-dimer (r=0.838; P<0.05). The receiver operating characteristic (ROC) area under the curve (AUC) for plasma miR-221 was 0.823 (95% CI, 0.757-0.906) (P<0.05), compared with the AUC for D-dimer of 0.768 (95% CI, 0.727-0.853), the AUC for troponin I of 0.713 (95% CI, 0.646-0.868), and the AUC for BNP of 0.648 (95% CI, 0.601-0.723). CONCLUSIONS Plasma levels of miR-221 were significantly increased in patients with acute PE when compared with healthy individuals.
本研究旨在测量和比较急性肺栓塞（PE）患者与健康个体的血浆中微小 RNA（miRNA）miR-221 的水平，并评估 miR-221 作为急性 PE 诊断生物标志物的潜在作用。
在采集的 60 例急性 PE 患者和 50 例健康志愿者的血样中，使用 microRNA 微阵列鉴定血浆中的 microRNA 水平，并使用实时定量逆转录聚合酶链反应（qRT-PCR）检测 miR-221 的表达。使用自动免疫分析仪测定脑钠肽（BNP）和肌钙蛋白 I。使用酶联免疫吸附试验（ELISA）测定 D-二聚体水平。
在评估的 32 种差异表达的血浆 miRNA 中，与正常个体相比，急性 PE 患者的血浆中 miR-221 明显上调（P<0.05）。相关性分析表明，急性 PE 患者的血浆 miR-221 水平与 BNP（r=0.842，P<0.05）、肌钙蛋白 I（r=0.853；P<0.05）和 D-二聚体（r=0.838；P<0.05）水平呈正相关。血浆 miR-221 的受试者工作特征（ROC）曲线下面积（AUC）为 0.823（95%可信区间，0.757-0.906）（P<0.05），而 D-二聚体的 AUC 为 0.768（95%可信区间，0.727-0.853），肌钙蛋白 I 的 AUC 为 0.713（95%可信区间，0.646-0.868），BNP 的 AUC 为 0.648（95%可信区间，0.601-0.723）。
与健康个体相比，急性 PE 患者的血浆 miR-221 水平显著升高。
