Herzik Mark A, Wu Mengyu, Lander Gabriel C
Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA.
Nat Methods. 2017 Nov;14(11):1075-1078. doi: 10.1038/nmeth.4461. Epub 2017 Oct 9.
Nearly all single-particle cryo-EM structures resolved to better than 4-Å resolution have been determined using 300-keV transmission electron microscopes (TEMs). We demonstrate that it is possible to obtain reconstructions of macromolecular complexes of different sizes to better than 3-Å resolution using a 200-keV TEM. These structures are of sufficient quality to unambiguously assign amino acid rotameric conformations and identify ordered water molecules.
几乎所有分辨率优于4埃的单颗粒冷冻电镜结构都是使用300千伏透射电子显微镜（TEM）测定的。我们证明，使用200千伏TEM能够获得不同大小的大分子复合物的重建结构，分辨率优于3埃。这些结构质量足够高，能够明确指定氨基酸旋转异构体构象并识别有序水分子。
