Anderson K J, Gibbs R B, Cotman C W
Department of Psychobiology, University of California, Irvine 92717.
Neuroscience. 1988 Apr;25(1):19-25. doi: 10.1016/0306-4522(88)90004-8.
Embryonic habenular or striatal cholinergic tissues were transplanted to the hippocampal formation of adult rats. The connectivity of these grafts with the host hippocampal formation was analysed using acetylcholinesterase histochemistry and immunocytochemistry with a monoclonal antibody to choline acetyltransferase. Both graft types produced laminar arrangements of acetylcholinesterase-positive fibers in the hippocampal formation that closely resembled the native pattern of cholinergic innervation. In addition, graft-derived choline acetyltransferase-immunoreactive synapses were found in the host hippocampal formation. These synapses were formed on non-immunoreactive dendritic structures and were similar to the types of cholinergic synapses found in the hippocampal formation of normal animals. These data indicate that the cholinergic transmitter phenotype is a major determinant of whether a neuron will form typical cholinergic synapses with hippocampal targets.
将胚胎期的缰核或纹状体胆碱能组织移植到成年大鼠的海马结构中。利用乙酰胆碱酯酶组织化学和针对胆碱乙酰转移酶的单克隆抗体进行免疫细胞化学分析，研究这些移植组织与宿主海马结构之间的连接情况。两种移植类型均在海马结构中产生了乙酰胆碱酯酶阳性纤维的分层排列，与胆碱能神经支配的天然模式极为相似。此外，在宿主海马结构中发现了源自移植组织的胆碱乙酰转移酶免疫反应性突触。这些突触形成于非免疫反应性的树突结构上，且与正常动物海马结构中发现的胆碱能突触类型相似。这些数据表明，胆碱能递质表型是神经元是否会与海马靶点形成典型胆碱能突触的主要决定因素。
