Frotscher M, Schlander M, Léránth C
Cell Tissue Res. 1986;246(2):293-301. doi: 10.1007/BF00215891.
We report here on cholinergic neurons in the rat hippocampal formation that were identified by immunocytochemistry employing a monoclonal antibody against choline acetyltransferase (ChAT), the acetylcholine-synthesizing enzyme. In general, ChAT-immunoreactive cells were rare, but were observed in all layers of the hippocampus proper and fascia dentata with a preponderance in zones adjacent to the hippocampal fissure and in the part of CA1 bordering the subiculum. All immunoreactive cells found were non-pyramidal neurons. They were relatively small with round or ovoid perikarya, which gave rise to thin spine-free dendrites. These hippocampal neurons were very similar to ChAT-immunoreactive cells in the neocortex of the same animals but were quite different from cholinergic neurons in the basal forebrain, medial septal nucleus, and neostriatum, which were larger and more intensely immunostained. Electron-microscopic analysis of ChAT-immunoreactive cells in the hippocampus and fascia dentata revealed synaptic contacts, mainly of the asymmetric type, on cell bodies and smooth proximal dendrites. The nuclei of the immunoreactive cells exhibited deep indentations, which are characteristic for non-pyramidal neurons. Our results provide evidence for an intrinsic source of the hippocampal cholinergic innervation in addition to the well-established septo-hippocampal cholinergic projection.
我们在此报告大鼠海马结构中的胆碱能神经元，这些神经元通过免疫细胞化学方法得以鉴定，该方法使用了一种针对胆碱乙酰转移酶（ChAT，乙酰胆碱合成酶）的单克隆抗体。一般来说，ChAT免疫反应阳性细胞较为罕见，但在海马体本部和齿状回的各层均有观察到，在靠近海马裂的区域以及CA1区与下托相邻的部分更为多见。所有发现的免疫反应阳性细胞均为非锥体神经元。它们相对较小，胞体呈圆形或椭圆形，发出细的无棘树突。这些海马神经元与同一动物新皮层中的ChAT免疫反应阳性细胞非常相似，但与基底前脑、内侧隔核和新纹状体中的胆碱能神经元有很大不同，后者体积更大，免疫染色更强。对海马体和齿状回中ChAT免疫反应阳性细胞的电子显微镜分析显示，在细胞体和光滑的近端树突上存在突触联系，主要为不对称型。免疫反应阳性细胞的细胞核呈现出深深的凹陷，这是非锥体神经元的特征。我们的结果为海马胆碱能神经支配除了已确立的隔海马胆碱能投射之外还存在内在来源提供了证据。
