Calienni Maria Natalia, Feas Daniela Agustina, Igartúa Daniela Edith, Chiaramoni Nadia Silvia, Alonso Silvia Del Valle, Prieto Maria Jimena
Laboratorio de Biomembranas - GBEyB (IMBICE, CCT-La Plata, CONICET), Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal B1876BXD, Argentina.
Laboratorio de Biomembranas - GBEyB (IMBICE, CCT-La Plata, CONICET), Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal B1876BXD, Argentina.
Toxicol Appl Pharmacol. 2017 Dec 15;337:1-11. doi: 10.1016/j.taap.2017.10.003. Epub 2017 Oct 7.
This article reports novel results about nanotoxicological and teratogenic effects of the PAMAM dendrimers DG4 and DG4.5 in zebrafish (Danio rerio). Zebrafish embryos and larvae were used as a rapid, high-throughput, cost-effective whole-animal model. The objective was to provide a more comprehensive and predictive developmental toxicity screening of DG4 and DG4.5 and test the influence of their surface charge. Nanotoxicological and teratogenic effects were assessed at developmental, morphological, cardiac, neurological and hepatic level. The effect of surface charge was determined in both larvae and embryos. DG4 with positive surface charge was more toxic than DG4.5 with negative surface charge. DG4 and DG4.5 induced teratogenic effects in larvae, whereas DG4 also induced lethal effects in both zebrafish embryos and larvae. However, larvae were less sensitive than embryos to the lethal effects of DG4. The platform of assays proposed and data obtained may contribute to the characterization of hazards and differential effects of these nanoparticles.
本文报道了聚酰胺-胺（PAMAM）树枝状大分子DG4和DG4.5对斑马鱼（Danio rerio）的纳米毒理学和致畸作用的新结果。斑马鱼胚胎和幼体被用作一种快速、高通量、经济高效的全动物模型。目的是对DG4和DG4.5进行更全面和可预测的发育毒性筛选，并测试其表面电荷的影响。在发育、形态、心脏、神经和肝脏水平评估纳米毒理学和致畸作用。在幼体和胚胎中都确定了表面电荷的影响。表面带正电荷的DG4比表面带负电荷的DG4.5毒性更大。DG4和DG4.5在幼体中诱导致畸作用，而DG4在斑马鱼胚胎和幼体中也诱导致死作用。然而，幼体对DG4致死作用的敏感性低于胚胎。所提出的检测平台和获得的数据可能有助于表征这些纳米颗粒的危害和差异效应。
