杠柳甲醇提取物的抗蝎子毒液活性：组织病理学和生物化学证据。
Anti-scorpion venom activity of Thapsia garganica methanolic extract: Histopathological and biochemical evidences.
机构信息
Laboratory of Ecology and Environment, Department of Biology, PO Box 2390, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakesh 40000, Morocco.
Laboratory of Ecology and Environment, Department of Biology, PO Box 2390, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakesh 40000, Morocco; Polyvalent Laboratory of Research & Development LPVRD, Department of Biology, Polydisciplinary Faculty, University Sultan Moulay Slimane, Beni Mellal, Morocco.
出版信息
J Ethnopharmacol. 2018 Jan 30;211:340-347. doi: 10.1016/j.jep.2017.10.003. Epub 2017 Oct 6.
ETHNOPHARMACOLOGICAL RELEVANCE
Thapsia garganica, is a herbal medicine traditionally used as diuretic, emetic and purgative. It is also used as anti-scorpion venom in Morocco; however, its protective effects against scorpion venom remain elusive.
AIM OF THE STUDY
The present study was undertaken to evaluate anti-venom activity of T. garganica in vivo through histological and biochemical studies.
MATERIALS AND METHODS
Methanolic leaves extract of T. garganica was evaluated for anti-venom activity against buthus. occitanus under in vivo conditions. Histopathological and biochemical changes in envenomed and treated mice were also examined. Phytochemical screening was conducted to estimate the major constituents whereas DPPH, β -Carotene-linoleic acid and reducing power assays were performed to evaluate the anti-oxidant activity of T. garganica extract.
RESULTS
Methanolic leaves extract of T. garganica (2g/kg) increased the survival time (> 18h) of mice injected with lethal doses of B. occitanus venom, with remarkable recovery of histology damage. Furthermore T. garganica induced a significant decreased of biochemical markers of kidney, liver and heart function. Phytochemistry screening revealed the presence of phenolic compounds, flavonoids, tannins and steroids/terpenoids, which might explain the bioactivity of the extract. It was also shown that the extract has an exceptionally high antioxidant activity compared to well-known antioxidants used as standards.
CONCLUSION
The present study provides strong evidence that support the use of T. garganica as anti-scorpion venom in traditional medicine in Morocco. However, additional studies are required to isolate and identify the metabolites responsible for the activity.
民族药理学相关性
Thapsia garganica 是一种草药，传统上用作利尿剂、催吐剂和泻药。它在摩洛哥也被用作抗蝎子毒液的药物；然而，其对蝎子毒液的保护作用仍不清楚。
研究目的
本研究旨在通过组织学和生化研究评估 T. garganica 的抗蛇毒活性。
材料和方法
在体内条件下，评估了 T. garganica 的甲醇叶提取物对 Buthus. occitanus 的抗蛇毒活性。还检查了中毒和治疗小鼠的组织病理学和生化变化。进行了植物化学筛选以估计主要成分，而 DPPH、β-胡萝卜素-亚油酸和还原力测定法用于评估 T. garganica 提取物的抗氧化活性。
结果
T. garganica 的甲醇叶提取物（2g/kg）增加了注射致死剂量 B. occitanus 毒液的小鼠的存活时间（>18h），并显著恢复了组织学损伤。此外，T. garganica 诱导了肾功能、肝功能和心脏功能生化标志物的显著降低。植物化学筛选显示存在酚类化合物、类黄酮、单宁和甾体/三萜类化合物，这可能解释了提取物的生物活性。还表明，与用作标准的已知抗氧化剂相比，该提取物具有异常高的抗氧化活性。
结论
本研究为摩洛哥传统医学中使用 T. garganica 作为抗蝎子毒液提供了有力证据。然而，需要进一步的研究来分离和鉴定负责该活性的代谢物。
Zotero 插件