Rymaszewski Z, Yunker R L, Ashraf M, Park M, Subbiah M T
Department of Internal Medicine, University of Cincinnati Medical Center, Ohio 45267.
Am J Physiol. 1988 Jul;255(1 Pt 2):H160-8. doi: 10.1152/ajpheart.1988.255.1.H160.
This study shows that amniotic fluid enhances cholesterol esterification in arterial wall, as measured by in vitro assay of acyl-CoA:cholesterol acyltransferase (ACAT) activity and by incorporation of oleic acid to cholesteryl esters in cultured fetal aortas and smooth muscle cells. This property is mostly evident in the fraction of molecular weight greater than 100,000, and it is abolished by delipidation, indicating that stimulating factor is probably lipoprotein in nature. Despite an increased cholesterol esterification by the presence of amniotic fluid in medium of cultured fetal aortas, the content of cholesterol and cholesteryl esters was much lower. The cellular structures are better preserved in explants cultured with amniotic fluid than in control animals. This study indicates that amniotic fluid contains factors that may have a pronounced effect on arterial wall during development.
本研究表明，羊水可增强动脉壁中的胆固醇酯化作用，这通过体外测定酰基辅酶A：胆固醇酰基转移酶（ACAT）活性以及通过在培养的胎儿主动脉和平滑肌细胞中将油酸掺入胆固醇酯中来衡量。这种特性在分子量大于100,000的部分中最为明显，并且通过脱脂作用被消除，表明刺激因子可能本质上是脂蛋白。尽管在培养的胎儿主动脉培养基中存在羊水会增加胆固醇酯化作用，但胆固醇和胆固醇酯的含量要低得多。与对照动物相比，用羊水培养的外植体中的细胞结构保存得更好。本研究表明，羊水含有可能在发育过程中对动脉壁产生显著影响的因子。
