Sikora Monika, Nowak Marcin, Racheniuk Henryk, Wojtysiak Katarzyna, Kozdrowski Roland
Wroclaw University of Environmental and Life Sciences, Faculty of Veterinary Medicine, Department of Reproduction and Clinic of Farm Animals, Plac Grunwaldzki Street 49, 50366, Wroclaw, Poland.
Folia Histochem Cytobiol. 2017;55(3):168-175. doi: 10.5603/FHC.a2017.0017. Epub 2017 Oct 10.
Endometritis and endometrosis have been and still are the major reasons for infertility in mares. The diagnosis of endometritis can be based on cytology and microbiology, but endometrial biopsy is still the only way to diagnose endometrosis in the mare. Our study attempted to determine if a single biopsy using his-topathology and immunohistochemistry is sufficient to ascertain reasons for infertility in Icelandic mares. The objectives of this study were to examine the relationship between deviations in endometrial biopsies in terms of prostaglandin-endoperoxide synthase-2 (PTGS-2) and fibronectin expression and polymorphonuclear cells (PMNs) infiltration, as well as scoring degeneration in two endometrial biopsies.
Materials were collected from 53 Icelandic breed mares, from whom two endometrial biopsies were collected and they were used for histopathology and for immunohistochemistry for PTGS-2 and fibronectin.
In our study, twenty-six of 53 mares (49%) showed differences in the biopsy score between the left and the right uterine horns (p = 0.002). There were statistically significant differences in fibronectin expression (p = 0.001), as well as in PTGS-2 expression in the superficial epithelium (p = 0.017).
Significant differences in the biopsy score, and fibronectin and PTGS-2 expression, between two endometrial biopsies obtained from individual mares demonstrated that a single biopsy could be insufficient for diagnosing uterine health status in Icelandic mares.
子宫内膜炎和子宫内膜异位过去是、现在仍然是母马不育的主要原因。子宫内膜炎的诊断可以基于细胞学和微生物学，但子宫内膜活检仍是诊断母马子宫内膜异位的唯一方法。我们的研究试图确定使用组织病理学和免疫组织化学进行单次活检是否足以确定冰岛母马不育的原因。本研究的目的是检查子宫内膜活检在前列腺素内过氧化物合酶-2（PTGS-2）和纤连蛋白表达以及多形核细胞（PMN）浸润方面的偏差之间的关系，以及对两次子宫内膜活检中的变性进行评分。
从53匹冰岛品种母马中收集材料，对每匹马收集两次子宫内膜活检样本，并将其用于组织病理学检查以及PTGS-2和纤连蛋白的免疫组织化学检测。
在我们的研究中，53匹母马中有26匹（49%）左右子宫角活检评分存在差异（p = 0.002）。纤连蛋白表达（p = 0.001）以及表层上皮中PTGS-2表达（p = 0.017）存在统计学显著差异。
从个体母马获得的两次子宫内膜活检在活检评分、纤连蛋白和PTGS-2表达方面存在显著差异，这表明单次活检可能不足以诊断冰岛母马的子宫健康状况。
