College of Landscape Architecture and Arts, Northwest A&F University, Yangling, Shaanxi 712100, China.
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Molecules. 2017 Oct 10;22(10):1694. doi: 10.3390/molecules22101694.
Lysophosphatidic acid acyltransferases (LPAATs) are essential for the acylation of lysophosphatidic acid (LPA) and the synthesis of phosphatidic acid (PA), a key intermediate in the synthesis of membrane phospholipids and storage lipids. Here, a putative lysophosphatidic acid acyltransferase gene, designated , was isolated from seed unsaturated fatty acid (UFA)-rich . The complete cDNA contained a 1116-bp open reading frame (ORF), encoding a 42.9 kDa protein with 371 amino acid residues. Bioinformatic analysis indicates that PrLPAAT4 is a plasma membrane protein belonging to acyl-CoA:1-acylglycerol-sn-3-phosphate acyltranferases (AGPAT) family. PrLPAAT4 shared high sequence similarity with its homologs from , , , and . In Arabidopsis, overexpression of resulted in a significant increase in the content of oleic acid (OA) and total fatty acids (FAs) in seeds. , , and , involved in TAG assembly, were upregulated in -overexpressing lines. These results indicated that PrLPAAT4 functions may be as a positive regulator in seed FA biosynthesis.
溶血磷脂酸酰基转移酶（LPAATs）对于溶血磷脂酸（LPA）的酰化和磷脂酸（PA）的合成至关重要，PA 是膜磷脂和储存脂质合成的关键中间产物。本文从富含不饱和脂肪酸（UFA）的种子中分离到一个假定的溶血磷脂酸酰基转移酶基因，命名为 。完整的 cDNA 包含一个 1116bp 的开放阅读框（ORF），编码一个 42.9kDa 的蛋白质，包含 371 个氨基酸残基。生物信息学分析表明，PrLPAAT4 是一种质膜蛋白，属于酰基辅酶 A：1-酰基甘油-3-磷酸酰基转移酶（AGPAT）家族。PrLPAAT4 与来自 、 、 和 的同源物具有很高的序列相似性。在拟南芥中， 过表达导致种子中油酸（OA）和总脂肪酸（FAs）含量显著增加。参与 TAG 组装的 、 和 在 -过表达系中上调。这些结果表明 PrLPAAT4 的功能可能作为种子 FA 生物合成的正调控因子。
