使用液体扫描电子显微镜对水中勃姆石颗粒进行原位表征。
In Situ Characterization of Boehmite Particles in Water Using Liquid SEM.
作者信息
Yao Juan, Arey Bruce W, Yang Li, Zhang Fei, Komorek Rachel, Chun Jaehun, Yu Xiao-Ying
机构信息
Earth & Biological Sciences Directorate, Pacific Northwest National Laboratory.
Earth & Biological Sciences Directorate, Pacific Northwest National Laboratory;
出版信息
J Vis Exp. 2017 Sep 27(127):56058. doi: 10.3791/56058.
In situ imaging and elemental analysis of boehmite (AlOOH) particles in water is realized using the System for Analysis at the Liquid Vacuum Interface (SALVI) and Scanning Electron Microscopy (SEM). This paper describes the method and key steps in integrating the vacuum compatible SAVLI to SEM and obtaining secondary electron (SE) images of particles in liquid in high vacuum. Energy dispersive x-ray spectroscopy (EDX) is used to obtain elemental analysis of particles in liquid and control samples including deionized (DI) water only and an empty channel as well. Synthesized boehmite (AlOOH) particles suspended in liquid are used as a model in the liquid SEM illustration. The results demonstrate that the particles can be imaged in the SE mode with good resolution (i.e., 400 nm). The AlOOH EDX spectrum shows significant signal from the aluminum (Al) when compared with the DI water and the empty channel control. In situ liquid SEM is a powerful technique to study particles in liquid with many exciting applications. This procedure aims to provide technical know-how in order to conduct liquid SEM imaging and EDX analysis using SALVI and to reduce potential pitfalls when using this approach.
利用液体真空界面分析系统（SALVI）和扫描电子显微镜（SEM）实现了对水中勃姆石（AlOOH）颗粒的原位成像和元素分析。本文描述了将真空兼容的SAVLI与SEM集成以及在高真空中获取液体中颗粒的二次电子（SE）图像的方法和关键步骤。能量色散X射线光谱（EDX）用于对液体中的颗粒以及仅包括去离子（DI）水和空通道的对照样品进行元素分析。悬浮在液体中的合成勃姆石（AlOOH）颗粒用作液体SEM示例中的模型。结果表明，颗粒可以在SE模式下以良好的分辨率（即400 nm）成像。与去离子水和空通道对照相比，AlOOH的EDX光谱显示出来自铝（Al）的显著信号。原位液体SEM是研究液体中颗粒的强大技术，具有许多令人兴奋的应用。本程序旨在提供技术诀窍，以便使用SALVI进行液体SEM成像和EDX分析，并减少使用此方法时的潜在陷阱。
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