牛黄体中的心房利钠肽。

Atrial natriuretic peptide in bovine corpus luteum.

作者信息

Vollmar A M, Mytzka C, Arendt R M, Schulz R

机构信息

Institute of Pharmacology, Toxicology, and Pharmacy, University of Munich, West Germany.

出版信息

Endocrinology. 1988 Aug;123(2):762-7. doi: 10.1210/endo-123-2-762.

DOI:10.1210/endo-123-2-762

PMID:2899502

Abstract

Atrial natriuretic peptide (ANP) has been demonstrated to exert endocrine functions, including the modulation of steroid synthesis. This prompted investigations to search for ANP receptors in the corpus luteum, a tissue that produces progesterone. The studies revealed a single binding site for [125I] ANP with similar characteristics (Kd, 122 pM; maximum binding, 18 fmol/mg protein) in all four stages of corpus luteum development. These receptors were demonstrated to stimulate cGMP production upon activation with synthetic ANP. Maximal cGMP synthesis was observed at 10(-7) M ANP, 5 min after activation of receptors. An acidic extract of corpus luteum contained immunoreactive ANP (approximately 220 fmol/g tissue), as indicated by gel chromatography, HPLC, and identification by means of a highly specific ANP antibody. The data do not permit definition of a specific endocrine role of ANP in the corpus luteum.

摘要

心房利钠肽（ANP）已被证明具有内分泌功能，包括调节类固醇合成。这促使人们研究在黄体（一种产生孕酮的组织）中寻找ANP受体。研究发现，在黄体发育的所有四个阶段，[125I]ANP都有一个单一的结合位点，其特征相似（解离常数Kd为122皮摩尔；最大结合量为18飞摩尔/毫克蛋白质）。这些受体被证明在与合成ANP激活后可刺激环磷酸鸟苷（cGMP）的产生。在受体激活5分钟后，在10^(-7) M的ANP浓度下观察到最大的cGMP合成。凝胶色谱、高效液相色谱以及通过高度特异性的ANP抗体鉴定表明，黄体的酸性提取物中含有免疫反应性ANP（约220飞摩尔/克组织）。这些数据无法明确ANP在黄体中的特定内分泌作用。