Vollmar A M, Mytzka C, Arendt R M, Schulz R
Institute of Pharmacology, Toxicology, and Pharmacy, University of Munich, West Germany.
Endocrinology. 1988 Aug;123(2):762-7. doi: 10.1210/endo-123-2-762.
Atrial natriuretic peptide (ANP) has been demonstrated to exert endocrine functions, including the modulation of steroid synthesis. This prompted investigations to search for ANP receptors in the corpus luteum, a tissue that produces progesterone. The studies revealed a single binding site for [125I] ANP with similar characteristics (Kd, 122 pM; maximum binding, 18 fmol/mg protein) in all four stages of corpus luteum development. These receptors were demonstrated to stimulate cGMP production upon activation with synthetic ANP. Maximal cGMP synthesis was observed at 10(-7) M ANP, 5 min after activation of receptors. An acidic extract of corpus luteum contained immunoreactive ANP (approximately 220 fmol/g tissue), as indicated by gel chromatography, HPLC, and identification by means of a highly specific ANP antibody. The data do not permit definition of a specific endocrine role of ANP in the corpus luteum.
心房利钠肽（ANP）已被证明具有内分泌功能，包括调节类固醇合成。这促使人们研究在黄体（一种产生孕酮的组织）中寻找ANP受体。研究发现，在黄体发育的所有四个阶段，[125I]ANP都有一个单一的结合位点，其特征相似（解离常数Kd为122皮摩尔；最大结合量为18飞摩尔/毫克蛋白质）。这些受体被证明在与合成ANP激活后可刺激环磷酸鸟苷（cGMP）的产生。在受体激活5分钟后，在10^(-7) M的ANP浓度下观察到最大的cGMP合成。凝胶色谱、高效液相色谱以及通过高度特异性的ANP抗体鉴定表明，黄体的酸性提取物中含有免疫反应性ANP（约220飞摩尔/克组织）。这些数据无法明确ANP在黄体中的特定内分泌作用。
