Okamura H, Kelly P A, Chabot J G, Morel G, Belles-Isles M, Heisler S
Laboratory of Molecular Endocrinology, McGill University, Royal Victoria Hospital, Montreal, QC, Canada.
Biochem Biophys Res Commun. 1988 Oct 31;156(2):1000-6. doi: 10.1016/s0006-291x(88)80943-4.
Binding activities and metabolic effects of atrial natriuretic peptide (ANP) were examined in rat brown fat. ANP binding sites were identified in thin sections of brown adipose. Binding of [125I]-ANP to brown fat membranes was specific, saturable and time-dependent. A single class of high affinity sites (Kd, 1.7 nM; Bmax, 226 fmol/mg protein) was present. ANP increased cGMP synthesis in isolated cells (EC50, 2.5 x 10(-9) M), but did not alter epinephrine-stimulated glycerol production. The results demonstrate the presence of specific ANP receptors in brown fat, activation of which increases cGMP formation; however, since neither ANP nor cGMP affect lipolysis, the biological importance of these receptors remains unknown.
