Ben-Shlomo R, Figueroa F, Klein J, Nevo E
Institute of Evolution, University of Haifa, Israel.
Genetics. 1988 May;119(1):141-9. doi: 10.1093/genetics/119.1.141.
Restriction fragment length polymorphisms (RFLPs) of two major histocompatibility class II genes (P alpha 1 and Q beta) were studied in 13 populations of four chromosomal species (2n = 52, 54, 58 and 60) of the mole rat, Spalax ehrenbergi superspecies in Israel. A substantial frequency of allelic fragments was found in both genes for all populations, including a desert isolate. In the P alpha 1 gene, one allelic fragment is a result of a deletion mutation which is diagnostic of the 2n = 52 chromosomal species. All other ten allelic variants are the result of point mutations. All mutations are located in a short region flanking the 3' end of the gene. Based on Mhc polymorphisms we confirm earlier evidence that gene flow does not occur between the older chromosomal species (2n = 52, 54, 58), and that reproductive isolation decreases, progressively from the oldest to the youngest species (2n = 60).
在以色列的埃氏鼹形鼠超种Spalax ehrenbergi的四个染色体物种（2n = 52、54、58和60）的13个种群中，研究了两个主要组织相容性复合体II类基因（Pα1和Qβ）的限制性片段长度多态性（RFLP）。在包括一个沙漠隔离种群在内的所有种群的这两个基因中，都发现了相当频率的等位基因片段。在Pα1基因中，一个等位基因片段是由一个缺失突变产生的，该突变可诊断2n = 52的染色体物种。所有其他十个等位基因变体都是点突变的结果。所有突变都位于基因3'端侧翼的一个短区域内。基于Mhc多态性，我们证实了早期的证据，即较古老的染色体物种（2n = 52、54、58）之间不存在基因流动，并且生殖隔离从最古老的物种到最年轻的物种（2n = 60）逐渐降低。
